Recombinant Human ST3GAL2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7275-GT

R&D Systems, part of Bio-Techne
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7275-GT-020
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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

CHO

Accession #

NP_008858

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 protein
Pro52-Gln350, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly56

Predicted Molecular Mass

35 kDa

SDS-PAGE

30-40 kDa, reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to bovine B1-3 galactosyl-N-acetyl galactosamine.
The specific activity is >250 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7275-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). Similar to ST3GAL1 (2), ST3GAL2 is a type II membrane protein localized in the trans-Golgi network that can transfer sialic acid to the Gal beta1-3GalNAc structure to form NeuAc alpha2-3Gal beta1-3GalNAc found in terminal carbohydrate groups of particular glycoproteins, oligosaccharides and glycolipids (3). Unlike the ubiquitously expressed ST3GAL1 (4), ST3GAL2 is mainly expressed in heart, liver, skeletal muscle and various lymphoid tissues but not in brain and kidney (3). ST3GAL2 is responsible for the synthesis of monosialosyl globopentaosylceramide (MSGb5), also known as stage-specific embryonic antigen-4 (SSEA4) (5), a cell surface marker for testicular germ cell carcinoma, renal cell carcinoma, and mesenchymal stem cells (6). The enzymatic activity of recombinant human ST3GAL2 was determined using a phosphatase-coupled glycosyltransferase assay (7).

References

  1. Varki, A. (1999) Glycobiology 2:25.
  2. Jeanneau, C. et al. (2004) J. Biol. Chem. 279:13461.
  3. Giordanengo, V. et al. (1997) Eur. J. Biochem. 247:558.
  4. Kitagawa, H. and Paulson, J.C. (1994) J. Biol. Chem. 269:17872.
  5. Saito, S. et al. (2003) J. Biol. Chem. 278:26474.
  6. Suila, H. et al. (2011) J. Mol. Cell Biol. 3:99.
  7. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 2

Alternate Names

Alpha 2,3-ST 2, Sialyltransferase 4B, SIAT4B, ST3GalA.2

Entrez Gene IDs

6483 (Human); 20444 (Mouse); 64442 (Rat)

Gene Symbol

ST3GAL2

UniProt

NP_008858

Additional ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Products

Product Documents for Recombinant Human ST3GAL2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human ST3GAL2 Protein, CF

For research use only

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