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Recombinant Human ST6GAL2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8330-GT

R&D Systems, part of Bio-Techne
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8330-GT-025

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ST6 Gal Sialyltransferase 2/ST6GAL2 protein
Glu243-Ser529, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu243

Predicted Molecular Mass

33 kDa

SDS-PAGE

37-43 kDa, reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum.
The specific activity is >0.45 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8330-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ST6 Gal Sialyltransferase 2/ST6GAL2

Sialic acid attached to glycoproteins or glycolipids plays important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion (1). Sialyltransferases are key enzymes in the biosynthesis of these sialoglycoconjugates. Human has two beta-galactoside alpha2,6-sialyltransferases, ST6GAL1 and ST6GAL1, with 48.9% amino acid sequence identity (2). While ST6GAL1 is expressed in almost all human tissues, ST6GAL2 shows a restricted tissue-specific pattern of expression, mostly in embryonic and adult brain (3). While ST6GAL1 has broad substrate specificity, ST6GAL2 catalyzes 2,6-sialylation of non-reducing end Gal beta1-4GlcNAc disaccharide on oligosaccharides and exhibits relatively low or no activities toward glycoproteins and glycolipids (4). Bovine ST6GAL2 prefers the disaccharide GalNAc beta1-4GlcNAc than the disaccharide Gal beta1-4GlcNAc (5).

References

  1. Varki, A. et al. (1999) Essentials of Glycobiology, ColdSpring Harbor Laboratory Press, pp195.
  2. Takashima, S. et al. (2002) J. Biol. Chem. 277:45719.
  3. Lehoux, S. et al. (2009) Glycoconj. J. 27:99.
  4. Takashima, S. et al. (2003) J. Biochem. 134:287.
  5. Laporte, B. et al. (2009) Glycobiology 19:1082.

Long Name

ST6 beta-Galactoside alpha-2,6-Sialyltransferase 2

Alternate Names

Alpha 2,6-ST 2, KIAA1877, Sialyltransferase 2, SIAT2

Entrez Gene IDs

84620 (Human); 240119 (Mouse); 301155 (Rat)

Gene Symbol

ST6GAL2

UniProt

Additional ST6 Gal Sialyltransferase 2/ST6GAL2 Products

Product Documents for Recombinant Human ST6GAL2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ST6GAL2 Protein, CF

For research use only

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