Recombinant Human ST6GALNAC6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7425-GT

R&D Systems, part of Bio-Techne
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7425-GT-010
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Key Product Details

Source

CHO

Accession #

Q969X2

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ST6 Sialyltransferase 6/ST6GALNAC6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ST6 Sialyltransferase 6/ST6GALNAC6 protein
Asn65-Thr333, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn65

Predicted Molecular Mass

32 kDa

SDS-PAGE

38-55 kDa, reducing conditions

Activity

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum.
The specific activity is >150 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7425-GT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ST6 Sialyltransferase 6/ST6GALNAC6

Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues (1). They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated by various sialyltransferases (2). ST6GALNAC6 is widely expressed in the nervous tissues and many other mouse tissues such as colon, liver, and heart. It has known activity on gangliosides of GD1a, GT1b and GM1b (3). ST6GALNAC6 is also responsible for the biosynthesis of disialylgalactosylgloboside and disialyl Lewis a, representative tumor-associated antigens in pancreas and colon cancers (4, 5). The recombinant ST6GALNAC6 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method (6) suggesting that ST6GALNAC6 is also active on N- or O-glycans.

References

  1. Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
  2. Harduin-Lepers, A. et al. Glycobiology 15:805.
  3. Okajima, T. et al. (2000) J. Biol. Chem. 275:6717.
  4. Senda, M. et al.(2007) Biochem J. 402:459.
  5. Tsuchida, A. et al. (2003) J. Biol. Chem. 278:22787.
  6. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Alternate Names

SIAT7F, ST6GALNACVI

Entrez Gene IDs

30815 (Human); 50935 (Mouse)

Gene Symbol

ST6GALNAC6

UniProt

Q969X2

Additional ST6 Sialyltransferase 6/ST6GALNAC6 Products

Product Documents for Recombinant Human ST6GALNAC6 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human ST6GALNAC6 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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