Recombinant Human Stabilin-2 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11086-S2

R&D Systems, part of Bio-Techne
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11086-S2-050
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Key Product Details

Source

CHO

Accession #

Q8WWQ8.3

Conjugate

Unconjugated

Applications

Bioactivity

View all Stabilin-2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Stabilin-2 protein
Ser20-Thr2458, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser20

Predicted Molecular Mass

265 kDa

SDS-PAGE

260-300 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Stabilin-2 His-tag Protein is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Hyaluronan binds with an ED50 of 1.50-7.50 ng/mL.

Scientific Data Images for Recombinant Human Stabilin-2 His-tag Protein, CF

Recombinant Human Stabilin‑2 His-tag Protein Binding Activity.

When Recombinant Human Stabilin-2 His-tag Protein (Catalog # 11086-S2) is immobilized at 2.00 μg/mL (100 μL/well), Biotinylated Hyaluronan binds with an ED50 of 1.50-7.50 ng/mL.

Recombinant Human Stabilin‑2 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Stabilin‑2 His-tag Protein (Catalog # 11086-S2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 260-300 kDa.

Formulation, Preparation and Storage

11086-S2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stabilin-2

Stabilin­-2, also known as HARE (Hyaluronic Acid Receptor for Endocytosis) and FEEL­2 (Fasciclin, EGF­-like, laminin type EGF­-like, and Link domain containing scavenger receptor 2), is a type I transmembrane multi­domain protein that is the closest homolog of stabilin­1. It is a scavenger receptor that is expressed on sinusoidal endothelial cells of liver, spleen, and lymph node (1, 2). Its 2439 amino acid (aa) extracellular domain contains seven fasciclin domains, multiple EGF-­like and laminin type EGF-­like domains, and a link domain related to molecules of the TSG­6 superfamily (3). The 72 aa cytoplasmic tail of Stabilin­2 contains a motif that allows the AP2 classical cargo adaptor to direct cargo into clathrin­coated pits (4). Stabilin­2 also mediates the endocytosis of chondroitin and chondroitin sulfate, advanced glycosylation end­product (AGE), collagen N­terminal propeptides and acetylated LDL (4-7). Stabilin-2 has been found to regulate the cancer progression, and high expression of Stabilin-2 was reported to predict poor tumor prognosis in NSCLC (8).

References

  1. Politz, O. et al. (2002) Biochem. J. 362:155.
  2. Harris, E.N. and F. Cabral (2019) Biomolecules 9:273.
  3. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  4. Hansen, B. et al. (2005) Exp. Cell Res. 303:160.
  5. Harris, E.N. et al. (2004) J. Biol. Chem. 279:36201.
  6. Harris, E.N. et al. (2007) J. Biol. Chem. 282:2785.
  7. Tamura, Y. et al. (2003) J. Biol. Chem. 278:12613.
  8. Yong, J. et al. (2021) Bioengineered 12:3426.

Alternate Names

FEEL-2, FELE-2, FELL-2, FEX2, HARE, STAB2, Stabilin2

Entrez Gene IDs

55576 (Human); 192188 (Mouse); 282580 (Rat)

Gene Symbol

STAB2

UniProt

Q8WWQ8.3

Additional Stabilin-2 Products

Product Documents for Recombinant Human Stabilin-2 His-tag Protein, CF

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Product Specific Notices for Recombinant Human Stabilin-2 His-tag Protein, CF

For research use only

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