Recombinant Human Stanniocalcin 1/STC-1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9400-SO

R&D Systems, part of Bio-Techne
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9400-SO-050
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Key Product Details

Source

NS0

Accession #

P52823-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Stanniocalcin 1/STC-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Stanniocalcin 1/STC-1 protein
Thr18-Ala247, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr18

Predicted Molecular Mass

27 kDa

SDS-PAGE

26-43 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 20-120 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN) .

Formulation, Preparation and Storage

9400-SO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stanniocalcin 1/STC-1

Stanniocalcin 1 (STC-1) is a homodimeric glycoprotein hormone that is involved in calcium and phosphate homeostasis. It was originally identified in bony fishes, where elevation of calcium in serum causes the release of STC from the endocrine glands called the corpuscles of Stannius (1-3). In contrast to fish where expression is mainly in the corpuscles of Stannius, the STC-1 gene in mammals is widely expressed in tissues including thyroid, ovary, prostate, adrenal gland, muscle, intestine, kidney, heart and lung (4). Human STC-1 protein consists of a 16 amino acid (aa) propeptide region and a 214 aa STC-1 chain. The amino acid sequence of human mature STC-1 is 36% identical to that of human STC-2. STC-1 is highly homologous with mouse, sharing 95% amino acid identity. STC-1 inhibits the breakdown of PAPP-A (5), protects cancer cells from apoptosis (4), reduces tumor size of liver cancers (6), promotes osteoblast differentiation (7) and inhibits longitudinal bone growth directly at the growth plate (8). It is also a biomarker of brain and lung cancer progression (7, 8).

References

  1. Yoshiko, Y. and J.E. Aubin. (2004) Peptides 25:1663.
  2. Wendelaar Bonga S.E. and P.K. Pang (1991) Int. Rev. Cytol. 128:139.
  3. Wascher, R.A. et al. (2003) Clin. Cancer Res. 9:1427.
  4. ­Ohkouchi, S. et al. (2015) Clin. Med. Insights Circ. Respir. Pulm. Med. 9:91.
  5. Kloverpris S, et al. (2015) J Biol Chem. 290:21915.
  6. Yeung BH, et al. (2015) PLoS One. 10:e0139977.
  7. Su J, et al. (2015) Tumour Biol. 36:6333.
  8. Du YZ, et al. (2016) Tumour Biol. 37:5633.

Alternate Names

STC-1, STC1

Entrez Gene IDs

6781 (Human)

Gene Symbol

STC1

UniProt

P52823-1

Additional Stanniocalcin 1/STC-1 Products

Product Documents for Recombinant Human Stanniocalcin 1/STC-1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Stanniocalcin 1/STC-1 Protein, CF

For research use only

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