Recombinant Human SULT1C2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7458-ST

R&D Systems, part of Bio-Techne
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7458-ST-020
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Key Product Details

Source

E. coli

Accession #

O00338

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Cytosolic Sulfotransferase 1C2/SULT1C2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Cytosolic Sulfotransferase 1C2/SULT1C2 protein
Ala2-Leu296, with N-terminal 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Inconclusive. Protein identity confirmed by mass spec, His tag confirmed by anti-His Western analysis.

Predicted Molecular Mass

38 kDa

SDS-PAGE

34-37 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to 4-nitrophenol.
The specific activity is >45 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7458-ST
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Cytosolic Sulfotransferase 1C2/SULT1C2

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi-resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1C2 is mainly expressed in the gastrointestinal tract (stomach, duodenum, jejunum, ileum, colon, caecum and rectum), liver and kidneys, but not in the lungs (3). In contrast, SULT1C4, a sulfotransferase that is most closely related to SULT1C2 (4), is expressed at higher levels in fetal lung and kidney and at lower levels in fetal heart. So far, SULT1C2 is found to be active only on p-nitrophenol (3). The enzymatic activity of our recombinant human SULT1C2 was determined using a phosphatase-coupled assay (5).

References

  1. Falany, C. N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Hehonah, N. et al. (1999) Int J. Biochem. Cell. Biol. 31:869.
  4. Sakakibara, Y. et al. (1998) J. Biol. Chem. 273:33929.
  5. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

ST1C1, ST1C2, SULT1C1, SULT1C2

Entrez Gene IDs

6819 (Human); 316153 (Rat)

Gene Symbol

SULT1C2

UniProt

O00338 (Human)

Additional Cytosolic Sulfotransferase 1C2/SULT1C2 Products

Product Documents for Recombinant Human SULT1C2 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human SULT1C2 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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