Recombinant Human SULT1C4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7095-ST

R&D Systems, part of Bio-Techne
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7095-ST-020
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Key Product Details

Source

E. coli

Accession #

O75897

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Cytosolic Sulfotransferase 1C4/SULT1C4 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Cytosolic Sulfotransferase 1C4/SULT1C4 protein
Ala2-Phe302, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

36 kDa

SDS-PAGE

34-38 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to 1-Napthol.
The specific activity is >150 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7095-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij-35 and DTT.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Cytosolic Sulfotransferase 1C4/SULT1C4

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1C4, originally designated as SULT1C2 (3), is expressed at higher levels in fetal lung and kidney and at lower levels in fetal heart. The endogenous substrate for this enzyme is unknown. It shows activity towards p‑nitrophenol and the carcinogenic compound, N‑hydroxy-2-acetylamino-fluorene (N-OH-2AAF) (3, 4).

References

  1. Falany, C. N. (1997) FASEB J. 11:206.
  2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
  3. Sakakibara, Y. et al. (1998) J. Biol. Chem. 273:33929.
  4. Yoshinari, K. et al. (2001) J. Biochem. Mol. Toxicol. 15:67.

Alternate Names

ST1C4, SULT1C4

Entrez Gene IDs

27233 (Human)

Gene Symbol

SULT1C4

UniProt

O75897

Additional Cytosolic Sulfotransferase 1C4/SULT1C4 Products

Product Documents for Recombinant Human SULT1C4 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human SULT1C4 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

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