Recombinant Human TAFA3/FAM19A3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4677-TA

R&D Systems, part of Bio-Techne
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4677-TA-050
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Key Product Details

Source

E. coli

Accession #

Q7Z5A8

Conjugate

Unconjugated

Applications

Bioactivity

View all TAFA3/FAM19A3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human TAFA3/FAM19A3 protein
Ala31-Arg133

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala31

Predicted Molecular Mass

11 kDa

SDS-PAGE

10 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human TAFA3/FAM19A3, immobilized at 4 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Formulation, Preparation and Storage

4677-TA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAFA3/FAM19A3

TAFA3, also called FAM19A3, is a member of the FAM19/TAFA family of small secreted proteins (1). Human TAFA3 has a molecular weight of approximately 15 kDa. The mature protein is 103 amino acids (aa) in length and shares 93% aa sequence identity with the mouse and rat orthologs (1). Alternative exon splicing causes a reading frame shift at aa 89, creating a 169 aa isoform with an altered C-terminal region (1). TAFA3 is specifically expressed in the brain with the highest levels being detected in parietal cortex and basal ganglia (1). TAFA proteins display chemokine-like signatures and appear to be distantly related to CCL3/ MIP-1 alpha as they share a common CC motif (1). TAFA3 has been suggested to be involved in transmitting the photoperiodically-controlled Melatonin signal from the pars tuberalis (2). TAFA3 mRNA levels in the pars tuberalis vary between night and day and correspond to plasma Melatonin levels (2). Additionally, these day/night expression differences are not observed in mice that lack Melatonin type 1 receptors (2). Interestingly, in other brain regions, such as the hippocampus and the ventral posterior nucleus of the thalamus, TAFA3 expression does not appear to be mediated by Melatonin, suggesting expression of this protein is controlled by region-specific mechanisms (2).

References

  1. Tom Tang, Y. et al. (2004) Genomics 83:727.
  2. Fischer, C. et al. (2012) Gen. Comp. Endocrinol. 177:98.

Long Name

Family with Sequence Similarity 19, Member A3

Alternate Names

FAM19A3

Entrez Gene IDs

284467 (Human); 329731 (Mouse)

Gene Symbol

TAFA3

UniProt

Q7Z5A8 (Human)

Additional TAFA3/FAM19A3 Products

Product Documents for Recombinant Human TAFA3/FAM19A3 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human TAFA3/FAM19A3 Protein, CF

For research use only

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