Recombinant Human THOP1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3439-ZN

R&D Systems, part of Bio-Techne
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3439-ZN-010
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Key Product Details

Source

E. coli

Accession #

NP_003240

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Thimet Oligopeptidase/THOP1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Thimet Oligopeptidase/THOP1 protein
Lys2-Cys689, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

80 kDa

SDS-PAGE

80 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl)-OH or Mca-PLGPK(Dnp)-OH.
The specific activity is >250 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

3439-ZN
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Thimet Oligopeptidase/THOP1

Thimet Oligopeptidase/THOP1, also known as endopeptidase EC 3.4.24.15 (EP24.15), is a zinc peptidase of the M3 family that also includes neurolysin/EC 3.4.24.16 and mitochondrial intermediate peptidase (1). Widely expressed by mammalian tissues and reported to present in different subcellular locations, THOP1 is primarily a cytoplasmic enzyme. It is capable of hydrolyzing a number of bioactive peptides and peptides released by the proteosome, limiting antigenic presentation by MHC class I molecules (1-3). THOP1 also interacts with angiotensin II type I receptor and bradykinin B2 receptor (4). The optimal activity of the purified THOP1 may or may not require the presence of a reducing agent, depending upon the source of the enzyme and the purification method used.

References

  1. Barrett, A.J. and J.-M. Chen (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 352, Elsevier Academic Press, San Diego.
  2. Ray, K. et al. (2004) J. Biol. Chem. 279:20480.
  3. Saric, T. et al. (2004) J. Biol. Chem. 279:46723.
  4. Shivakumar, B.R. et al. (2005) Cell Biochem. Funct. 23:195.

Alternate Names

EP24.15

Entrez Gene IDs

7064 (Human); 50492 (Mouse); 64517 (Rat)

Gene Symbol

THOP1

UniProt

NP_003240

Additional Thimet Oligopeptidase/THOP1 Products

Product Documents for Recombinant Human THOP1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human THOP1 Protein, CF

For research use only

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