Recombinant Human Thrombospondin-3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8390-TH

R&D Systems, part of Bio-Techne
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8390-TH-050
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Key Product Details

Source

HEK293

Accession #

P49746

Structure / Form

Disulfide-linked oligomer

Conjugate

Unconjugated

Applications

Bioactivity

View all Thrombospondin-3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Thrombospondin-3 protein
Ser22-Val956 with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

103 kDa

SDS-PAGE

100-145 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.3-1.5 μg/mL.

Formulation, Preparation and Storage

8390-TH
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and CHAPS.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-3

Thrombospondin-3 (TSP-3) is a 140-150 kDa heparin-binding protein in the thrombospondin family of matricellular adhesion proteins. Within this family, TSP-3, TSP-4, and TSP-5/COMP are secreted as pentamers, while TSP-1 and TSP-2 are homotrimeric (1). TSP-3 contains an N-terminal Laminin G-like globular domain, a flexible linker region, multiple tandem EGF-like repeats, multiple tandem TSP type-3 repeats, and a globular TSP C-terminal domain (2). Alternative splicing of human TSP-3 generates a short isoform with a deletion of approximately half of the Laminin-like domain and a portion of the linker. Mature human TSP-3 shares 98% and 97% amino acid sequence identity with mouse and rat TSP-3, respectively. In the disulfide-linked TSP-3 pentamer, the Laminin-like domains are clustered while the C-terminal globular domains are extended (3). TSP-3 is expressed during mouse embryogenesis in the brain, dorsal root ganglia, cartilage, lung, and gut (4, 5). In postnatal mice it is most highly expressed in the lung (6). TSP-3 is involved in the columnar organization of chondrocytes in developing growth plates of long bones (7). It is up-regulated in metastatic osteosarcoma and in the corneal stroma during wound healing (8, 9).

References

  1. Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
  2. Adolph, K.W. et al. (1995) Genomics 27:329.
  3. Qabar, A. et al. (1995) J. Biol. Chem. 270:12725.
  4. Iruela-Arispe, M.L. et al. (1993) Dev. Dyn. 197:40.
  5. Qabar, A. et al. (1994) J. Biol. Chem. 269:1262.
  6. Vos, H.L. et al. (1992) J. Biol. Chem. 267:12192.
  7. Posey, K.L. et al. (2008) Am. J. Pathol. 172:1664.
  8. Dalla-Torre, C.A. et al. (2006) BMC Cancer 6:237.
  9. Armstrong, D.J. et al. (2002) Int. J. Biochem. Cell Biol. 34:588.

Alternate Names

THBS3, Thrombospondin3, TSP3

Entrez Gene IDs

7059 (Human); 21827 (Mouse); 681309 (Rat)

Gene Symbol

THBS3

UniProt

P49746

Additional Thrombospondin-3 Products

Product Documents for Recombinant Human Thrombospondin-3 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Thrombospondin-3 Protein, CF

For research use only

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