Recombinant Human Thymidine Kinase 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8180-TK

R&D Systems, part of Bio-Techne
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8180-TK-050
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Key Product Details

Source

E. coli

Accession #

P04183

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Thymidine Kinase 1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Thymidine Kinase 1 protein
Ser2-Asn234, with C-terminal 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2 & Cys3

Predicted Molecular Mass

26 kDa

SDS-PAGE

25-30 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate thymidine.
The specific activity is >550 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8180-TK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol, Brij-30 and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Thymidine Kinase 1

Thymidine kinases are 2'-deoxythymidine kinases that phosphorylate deoxythymidine and generate deoxythymidine 5’-phosphate (1). Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Two forms of thymidine kinase are present in mammalian cells, TK1 and TK2. TK1 is cell cycle-dependent and is present in the cytoplasm only in anticipation of cell division (2, 3); whereas TK2 is located in mitochondria and is cell cycle-independent (4). TK1 is synthesized by the cell during the S phase of cell division and is degraded after cell division is completed (5). TK1 normally occurs in tissue as a dimer and can be converted to more active tetrameric form by ATP binding (6). TK1 is feedback inhibited by thymidine triphosphate, the product of the further phosphorylation of thymidine (7). Because tumor cells replicate much more frequently than normal cells and requires faster DNA synthesis and higher TK1 activity, TK1 is a cancer maker especially for hematologic malignancies (8, 9). In clinical chemistry TK1 is used as a proliferation marker in the diagnosis, control of treatment and follow-up of malignant disease (10). In addition, thymidine kinase is required for the action of many antiviral drugs (11). The enzymatic activity of recombinant human TK1 is measured using a phosphatase-coupled method (12).

References

  1. Wintersberger, E. (1997) Biochem. Soc. Trans. 25:303.
  2. Littelfield, J.W. (1966) Biochim. Biophys. Acta 114:398.
  3. Flemington, E, et al. (1987) Gene 52:267.
  4. Berk, A.J. et al. (1973) Arch. Biochem. Biophys. 154:563.
  5. Zhu, C. et al. (2006) Nucleosides Nucleotides Nucleic Acids 25:1185.
  6. Munch-Petersen, B. et al. (1993) J. Biol. Chem. 268:15621.
  7. Mikkelsen, N. E. et al. (2003). Biochemistry 42:5706.
  8. Doi S, et al. (1990) Nagoya J. Med. Sci. 52:19.
  9. Ellims PH, et al. (1981). Cancer Res. 41: 691.
  10. Lin TS, et al. (1976) J. Med. Chem.19:495.
  11. Baba M, et al. (1987). Biochem. Biophys. Res. Commun. 142:128.
  12. Wu, Z.L. (2011) PLoS One 6:e23172.

Alternate Names

TK1

Entrez Gene IDs

7083 (Human); 21877 (Mouse)

Gene Symbol

TK1

UniProt

P04183

Additional Thymidine Kinase 1 Products

Product Documents for Recombinant Human Thymidine Kinase 1 Protein, CF

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