Recombinant Human TMED1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2243-TM

R&D Systems, part of Bio-Techne
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2243-TM-050
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Key Product Details

Source

HEK293

Accession #

Q13445

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TMED1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human TMED1 protein
Human TMED1
(Ala24-Asn194)
Accession # Q13445		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala24

Predicted Molecular Mass

46 kDa

SDS-PAGE

56-62 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human TMED1 Fc Chimera is coated at 0.5 µg/mL, 100 μL/well, Recombinant Human ST2/IL‑1 R4 Fc Chimera (Catalog # 523-ST) binds with an ED50 of 0.6-3.6 μg/mL

Scientific Data Images for Recombinant Human TMED1 Fc Chimera Protein, CF

Recombinant Human TMED1 Fc Chimera Protein Binding Activity

Recombinant Human TMED1 Fc Chimera Protein Binding Activity

When Recombinant TMED1 Fc Chimera (Catalog # 2243-TM) is coated at 0.5 µg/mL, Recombinant Human ST2/IL-1 R4 Fc Chimera (Catalog # 523-ST) binds with an ED50 of 0.6-3.6 µg/mL.
Recombinant Human TMED1 Fc Chimera Protein SDS-PAGE

Recombinant Human TMED1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human TMED1 Fc was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 56-62 kDa and 110-120 kDa, respectively.

Formulation, Preparation and Storage

2243-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: TMED1

TMED1 (Transmembrane Emp24 domain-containing protein 1) is a member of the TMED family of proteins (gene name TMED1). The TMED family of proteins are localized to membranes of the early secretory pathway, including the endoplasmic reticulum and Golgi, and function in vesicular protein trafficking (1, 2). TMED1 is a 59 kDa monomer and has been reported to exist as homodimer (3). TMED1 is composed of a 23 amino acid (aa) signal sequence, a 171 aa extra cellular domain, a 21 aa transmembrane domain, and a 12 aa cytoplasmic domain. The extracellular domain contains an 83 aa GOLD (Golgi Dynamics) domain, and COPI and COPII binding motifs are found in the cytoplasmic domain (1-3, 5). Human TMED1 shares 97% sequence identity with mouse, bovine, and rat homologs within the 171 aa extracellular domain. The beta-strand-rich GOLD domain has been specifically identified to be involved in intracellular protein trafficking (1, 4, 5). TMED1 is important in regulating innate immune signaling through its interaction with ST2L. Specifically, the GOLD domain in TMED1 interacts with the TIR domain of ST2L, a receptor for IL-33 (1). This interaction promotes ST2L association with IL-33, allowing downstream signaling cascade activating MAP kinases, p38, and JNK (1, 6). Studies have shown knockdown of TMED-1 in HUVECs impairs the IL-33 induced response resulting in reduction of IL-6 and IL-8 productions (1).

References

  1. Connolly, D. et al. (2013) J Biol Chem. 288:5616.
  2. Gour, N. and Lajoie, S. (2018) Curr Allergy Ashma Rep. 16:65.
  3. Jenne, N. (2002) J Biol Chem. 277:46504.
  4. Anantharaman, V. and Aravind, L. (2002) Genome Biol. 3:research0023
  5. Gomez-Navarro, N. and Miller, E. (2016) J Cell Biol. 215:769.
  6. Hardman, C. and Ogg, G. (2016). Curr Opin Immunol. 42:16.

Alternate Names

IL-1RL1LG, IL1RL1-Binding Protein, Il1rl1l, IL1RL1LG, Interleukin 1 Receptor-Like 1 Ligand, Ly84l, P24g1, P24gamma1, Tp24

Entrez Gene IDs

11018 (Human); 17083 (Mouse); 315461 (Rat)

Gene Symbol

TMED1

UniProt

Q13445

Additional TMED1 Products

Product Documents for Recombinant Human TMED1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human TMED1 Fc Chimera Protein, CF

For research use only

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