Recombinant Human TMEFF2/Tomoregulin-2 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11107-TF

R&D Systems, part of Bio-Techne
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11107-TF-050
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Key Product Details

Source

NS0

Accession #

Q9UIK5.1

Conjugate

Unconjugated

Applications

Bioactivity

View all TMEFF2/Tomoregulin-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TMEFF2/Tomoregulin-2 protein
Arg34-Val320, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg34

Predicted Molecular Mass

33 kDa

SDS-PAGE

55-66 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PDGF-AA  (Catalog # 221-AA) is immobilized at 5.00 μg/mL (100 μL/well), Recombinant Human TMEFF2/Tomoregulin-2 His-tag Protein binds with an ED50 of 0.750-4.50 μg/mL.

Scientific Data Images for Recombinant Human TMEFF2/Tomoregulin-2 His-tag Protein, CF

Recombinant Human TMEFF2/Tomoregulin‑2 His-tag Protein Binding Activity.

When Recombinant Human PDGF-AA (221-AA) is immobilized at 5.00 μg/mL (100 μL/well), Recombinant Human TMEFF2/Tomoregulin-2 His-tag Protein (Catalog # 11107-TF) binds with an ED50 of 0.750-4.50 μg/mL.

Recombinant Human TMEFF2/Tomoregulin‑2 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human TMEFF2/Tomoregulin‑2 His-tag Protein (Catalog # 11107-TF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-66 kDa.

Formulation, Preparation and Storage

11107-TF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEFF2/Tomoregulin-2

TMEFF2 (transmembrane protein with an EGF-like and two Follistatin-like domains 2), also known as Tomoregulin-2, is a type I transmembrane glycoprotein that contains two extracellular follistatin modules, an extracellular EGF-like domain, a transmembrane domain, and a short conserved cytoplasmic tail (1) Soluble extracellular domains of both TMEFF1 and 2 can be generated by proteolytic cleavage. TMEFF2 amino acid (aa) sequences are 98.9% identical for human and mouse orthologs (1). The differential methylation of TMEFF2 can be related to the response to therapy and survival outcomes of the stages of cancers such as breast, prostate, lung, bladder, colon and rectal, gallbladder, renal, oesophageal, cardiac, stomach/gastric, ovarian, multiple myeloma, glioblastoma, and mesothelioma. The range of tissue expression of TMEFF2 functions as a possible biomarker and/or therapeutic target (1). Studies showed that TMEFF2 binds and inhibits PDGF-AA (2, 3). It also inhibits the proliferation of DU145 and PC3 prostate cancer cell lines (4). The amyloid-b protein, its precursor AbPP, and amyloid- b proteins AbPOs also bind TMEFF2, suggesting a neuroprotective role on Alzheimer's disease (5). TMEFF2's diverse function includes metabolism, embryonic development, cytoskeletal binding, extracellular matrix binding, chromatin binding and interaction of RNA polymerase II with DNA (6), and neuronal development (7).

References

  1. Masood, M. et al. (2020) Cancers 12:3862.
  2. Lin, K. et al. (2011) PLoS ONE 6:e18608.
  3. Huang, H. et al. (2017) Sci. Rep. 7:337.
  4. Gery, S. et al. (2002) Oncogene 21:4739.
  5. Hong, H.S. et al. (2015) Alzheimers Dis. 48:731.
  6. Gao, L. et al. (2020) Life Sci. 243:116910.
  7. Horie, M. et al. (2000) Genomics 67:146.

Long Name

Transmembrane Protein with EFG-like and two Follistatin-like Domains

Alternate Names

TENB2, Tomoregulin-2, Tomoregulin2

Entrez Gene IDs

23671 (Human); 56363 (Mouse)

Gene Symbol

TMEFF2

UniProt

Q9UIK5.1

Additional TMEFF2/Tomoregulin-2 Products

Product Documents for Recombinant Human TMEFF2/Tomoregulin-2 His-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human TMEFF2/Tomoregulin-2 His-tag Protein, CF

For research use only

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