Recombinant Human TMIGD2/CD28H Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI8316

Biotinylated Fc Chimera
R&D Systems, part of Bio-Techne
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AVI8316-050
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Key Product Details

Source

CHO

Accession #

Q96BF3.2

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all TMIGD2/CD28H Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TMIGD2/CD28H protein
Human TMIGD2/CD28H
(Leu23-Gly150)
Accession # Q96BF3.2		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

43 kDa

SDS-PAGE

57-64 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human B7-H7 Fc Chimera (Catalog # 8084-B7) is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human TMIGD2/CD28H Fc Chimera Avi-tag (Catalog # AVI8316) binds with an ED50 of 50.0-500 ng/mL

Scientific Data Images for Recombinant Human TMIGD2/CD28H Fc Avi-tag Protein, CF

Biotinylated Recombinant Human TMIGD2/CD28H Fc Chimera Avi-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H7 Fc Chimera (8084-B7) is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human TMIGD2/CD28H Fc Chimera Avi-tag Protein (Catalog # AVI8316) binds with an ED50 of 50.0-500 ng/mL

Biotinylated Recombinant Human TMIGD2/CD28H Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human TMIGD2/CD28H Fc Chimera Avi-tag Protein (Catalog # AVI8316) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-64 kDa and 114-122 kDa, respectively.

Formulation, Preparation and Storage

AVI8316
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5). Our Avi-tag Biotinylated human CD28H Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
  2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
  3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
  4. Huang, C. et al. (2003) Nature 424:219.
  5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.

Long Name

Transmembrane And Immunoglobulin Domain Containing 2

Alternate Names

CD28H, IGPR-1, IGPR1

Entrez Gene IDs

126259 (Human)

Gene Symbol

TMIGD2

UniProt

Q96BF3.2

Additional TMIGD2/CD28H Products

Product Documents for Recombinant Human TMIGD2/CD28H Fc Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human TMIGD2/CD28H Fc Avi-tag Protein, CF

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