Recombinant Human TPST2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6236-ST
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Tyrosylprotein Sulfotransferase 2/TPST2 protein
Gln26-Ser377, with a C-terminal 6-His tag
Gln26-Ser377, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No N-terminal sequence was revealed by sequencing. The Gln residue maybe blocked. Protein identity confirmed by MS analysis of tryptic fragments.
Predicted Molecular Mass
40 kDa
SDS-PAGE
40-50 kDa, reducing conditions
Activity
Measured by its ability to transfer sulfate from PAPS to PSGL-1 peptide (Gln-Ala-Thr-Glu-Tyr-Glu-Tyr-Leu-Asp-Tyr-Asp-Phe-Leu-Pro-Glu-Thr)
The specific activity is >20 pmol/min/μg, as measured under the described conditions.
The specific activity is >20 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
6236-ST
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Tyrosylprotein Sulfotransferase 2/TPST2
Tyrosine O-sulfation is a posttranslational modification found in all multicellular organisms (1, 2, 3). This reaction is mediated by tyrosylprotein sulfotransferase (TPST), a Golgi enzyme that transfers sulfate from 3'-phosphoadenosine 5'‑phosphosulfate (PAPS) to tyrosine residues contained in polypeptides with acidic motifs to form a tyrosine O-sulfate ester. More than 60 proteins have been identified to be tyrosine sulfated. The function of this modification have been identified in some cases. For example, sulfation of tyrosine residues in the leukocyte adhesion molecule P‑selectin glycoprotein ligand 1 (PSGL-1) is required for binding to P‑selectin on activated endothelium (4, 5), and tyrosine sulfation of chemokine receptors CCR5 and CXCR4 has been reported to facilitate HIV-1 entry of target cells (6, 7). Two TPSTs are found in the human genome. Compared to TPST1, TPST2 has similar tissue distribution and overlapping substrate specificity (8, 9). In contrast to
Tpst1-/- males with normal fertility in mice, Tpst2-/- males are infertile due to severe defects in sperm motility (10). The enzymatic activity was assayed using an SDS-PAGE based method (11).
References
- Kehoe, J.W. and Bertozzi, C.R. (2000) Chemistry & Biology 7:R57.
- Huttner, W.B. (1988) Annu. Rev. Physiol. 50:363.
- Ouyang, Y. et al. (1998) Proc. Natl. Acad. Sci. USA 95:2896.
- Danan, L.M. et al. (2008) J. Am. Soc. Mass. Spectrom. 19:1459.
- Somers, W.S. et al. (2000) Cell 103:467.
- Choe, H. et al. (2003) Cell 114:161.
- Seibert, C. et al. (2008) Biochemistry 47:11251.
- Ouyang, Y.B. and Moore, K.L. (1998) J. Biol. Chem. 273:24770.
- Jen, C.H. (2009) Biochemistry 48:5332.
- Borghei, A. et al. J. Biol. Chem. 281:9423.
- Wu, Z.L. et al. (2010) BMC Biotechnol. 10:11.
- Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
- MacRae, I.J. et al. (2000) Biochemistry 39:1613.
- Wu, Z.L. et al. (2002) FASEB J. 16:539.
Alternate Names
Tyrosylprotein Sulfotransferase 2
Entrez Gene IDs
8459 (Human)
Gene Symbol
TPST2
UniProt
Additional Tyrosylprotein Sulfotransferase 2/TPST2 Products
Product Documents for Recombinant Human TPST2 Protein, CF
Product Specific Notices for Recombinant Human TPST2 Protein, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd. Triton is a registered trademark of Union Carbide Corp. Glogos is a registered trademark of Stratagene.
For research use only
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