Recombinant Human TREML2/TLT-2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3259-TL

R&D Systems, part of Bio-Techne
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3259-TL-050
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Key Product Details

Source

NS0

Accession #

CAI19909

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all TREML2/TLT-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human TREML2/TLT-2 protein
Human TREML2
(Gly78 - Ser327)
Accession # CAI19909		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly78

Predicted Molecular Mass

53.7 kDa (monomer)

SDS-PAGE

75-100 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
Human PBMC cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with anti-CD3 and recombinant human TREML2/Fc Chimera.
The ED50 for this effect is 0.45-1.8 μg/mL.
Optimal dilutions should be determined by each laboratory for each applications.

Formulation, Preparation and Storage

3259-TL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML2/TLT-2

Trem-like transcript 2 protein (TLT-2; also TREML2) is a 33 kDa (unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Human TLT-2 is synthesized as a 321 amino acid (aa) precursor that contains an 18 aa signal sequence, a 250 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 32 aa cytoplasmic tail (SwissProt # Q5T2D2). Within the ECD are an Ig-like V-type domain (aa 20 ‑ 121) and one potential site of N-linked glycosylation. Mature human TLT-2 shares 49% aa sequence identity with mature mouse TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines (1 ‑ 2). TLT-2 is also expressed constitutively on CD8+ T cells and induced on CD4+ T cells after activation (2). TLT-2 plays a role in the innate and adaptive immune responses. In 2008, Hashiguchi et al. discovered that the B7 family member B7-H3, which also plays important roles in immune responses, specifically bound to TLT-2. According to their research, stimulation with B7-H3 transfectants preferentially up-regulated the proliferation and IFN-gamma production on CD8+ T cells (2). Also, transduction of TLT-2 into T cells resulted in enhanced IL-2 and IFN-gamma production via interactions with B7-H3, and blockade of the B7‑H3:TLT‑2 pathway with mAb against B7-H3 or TLT-2 efficiently inhibited contact hypersensitivity responses (2). In 2009, however, Leitner et al. extensively analyzed interaction of B7-H3 with TLT-2 and found no evidence for such an interaction (3).

References

  1. King, R.G. et al. (2006) J. Immunol. 176:6012.
  2. Hashiguchi, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:10495.
  3. Leitner, J. et al. (2009) Eur. J. Immunol. 39:1754.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 2

Alternate Names

TLT2

Entrez Gene IDs

79865 (Human); 328833 (Mouse)

Gene Symbol

TREML2

UniProt

CAI19909

Additional TREML2/TLT-2 Products

Product Documents for Recombinant Human TREML2/TLT-2 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human TREML2/TLT-2 Fc Chimera Protein, CF

For research use only

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