Recombinant Human TrkA Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11378

Biotinylated
R&D Systems, part of Bio-Techne
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AVI11378-050
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Key Product Details

Source

CHO

Accession #

P04629.4

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all TrkA Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TrkA protein
Human TrkA
(Ala34-Gly423)
Accession # P04629.4		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala34

Predicted Molecular Mass

71 kDa

SDS-PAGE

105-120 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human beta-NGF (Catalog # 256-GF) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human TrkA Fc Chimera Avi-tag  (Catalog # AVI11378) binds with an ED50 of 7.00-70.0 ng/mL.

Scientific Data Images for Recombinant Human TrkA Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human TrkA Fc Chimera Avi-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human beta-NGF (256-GF) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human TrkA Fc Chimera Avi-tag Protein (Catalog # AVI11378) binds with an ED50 of 7.00-70.0 ng/mL.

Biotinylated Recombinant Human TrkA Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human TrkA Fc Chimera Avi-tag Protein (Catalog # AVI11378) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-120 kDa and 210-240 kDa, respectively.

Formulation, Preparation and Storage

AVI11378
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Background: TrkA

TrkA (Tyrosine kinase receptor A), also known as High affinity NGF receptor, is a member of the neurotrophic tyrosine kinase receptor family that has three members,  TrkA, Trk B and Trk C, which preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively (1). All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues (1). NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily (2). NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when being expressed independent of Trk (3). Our Avi-tag Biotinylated human TrkA Fc Chimeria features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Shelton D.L. et al. (1995) J. Neurosci. 15:477.
  2. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  3. Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat); 102129364 (Cynomolgus Monkey)

Gene Symbol

NTRK1

UniProt

P04629.4

Additional TrkA Products

Product Documents for Recombinant Human TrkA Fc Chimera Avi-tag Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human TrkA Fc Chimera Avi-tag Protein, CF

For research use only

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