Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5047-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro and mature forms
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Tryptase epsilon/BSSP-4 protein
Ala33-Ser317 & Val50-Ser317, both with a C-terminal 10-His tag
Ala33-Ser317 & Val50-Ser317, both with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala33 & Val50
Predicted Molecular Mass
32 kDa
SDS-PAGE
Multiple bands between 33-38 kDa, reducing conditions
Activity
Measured by its ability to cleave benzyloxycarbonyl-Arg-thiobenzyl ester (Z-R-SBzl).
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
5047-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Tryptase epsilon/BSSP-4
Tryptase epsilon, also known as brain-specific protease-4 (BSSP-4) and prosemin, is expressed in the brain and epithelium-rich tissues such as the lung and eye (1, 2). BSSP-4 is a serine protease related to trypsin, preferentially hydrolyzing substrates after arginine and lysine residues. However, Tryptase epsilon is less susceptible to inhibition by common trypsin inhibitors such as aprotinin, alpha1-antitrypsin and secretory leukocyte protease inhibitor (3). Tryptase epsilon efficiently converts pro‑urokinase‑type plasminogen activator to its mature, active form (3).
References
- Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.
- Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
- Yasuda, S. et al. (2005) Blood 105:3893.
Alternate Names
BSP-2, BSSP-4, BSSP4, PRSS22
Gene Symbol
PRSS22
UniProt
Additional Tryptase epsilon/BSSP-4 Products
Product Documents for Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF
Product Specific Notices for Recombinant Human Tryptase epsilon/BSSP-4 Protein, CF
For research use only
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