Recombinant Human TSLPR Fc Chimera Alexa Fluor® 647 Protein
R&D Systems, part of Bio-Techne | Catalog # AFR981
Key Product Details
Learn more about FluorokinesTM Fluorescent-Labeled Proteins
Source
NS0
Accession #
Structure / Form
Disulfide linked homodimer.
Labeled with Alexa Fluor® 647 via amines.
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm
Labeled with Alexa Fluor® 647 via amines.
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm
Conjugate
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human TSLPR protein
| Human TSLPR (Gly25-Lys231) Accession # Q9HC73.1 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly25
Predicted Molecular Mass
51 kDa (monomer)
SDS-PAGE
65-75 kDa, reducing conditions.
Activity
Measured by flow cytometry for its ability to bind anti-human TSLPR Monoclonal Antibody conjugated beads.The concentration of Recombinant Human TSLPR Fc Chimera Alexa Fluor® 647 (Catalog # AFR981) that produces 50% of the binding response is 5.00-50.0 ng/mL
Scientific Data Images for Recombinant Human TSLPR Fc Chimera Alexa Fluor® 647 Protein
Flow cytometry analysis for Recombinant Human TSLPR Fc Chimera Alexa Fluor® 647 staining on anti-human TSLPR Monoclonal Antibody conjugated beads.
Streptavidin coated beads conjugated to biotinylated anti-human TSLPR Monoclonal Antibody were stained with the indicated concentrations of Recombinant Human TSLPR Fc Chimera Alexa Fluor® 647 (Catalog # AFR981).
Recombinant Human TSLPR Fc Chimera Alexa Fluor® 647 Protein SDS-PAGE.
2 μg/lane of Recombinant Human TSLPR Fc Chimera Alexa Fluor® 647 Protein (Catalog # AFR981) was resolved with SDS-PAGE under reducing (R) and nonreducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-75 kDa and 130-150 kDa, respectively.Formulation, Preparation and Storage
AFR981
| Formulation | Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: TSLPR
TSLPR, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLPR (3). The human TSLPR cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS-like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLPR displays 39% identity to mouse TSLPR and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLPR has also been reported in mouse (2). TSLPR expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLPR alone bind TSLP with low affinity. Co-expression of TSLPR and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3-6). The TSLPR and IL-7 R alpha are co-expressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).
References
- Fujio, K. et al. (2000) Blood 95:2204.
- Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
- Park, L.S. et al. (2000) J. Exp. Med. 192:659.
- Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
- Reche, P.A. et al. (2001) J. Immunol. 167:336.
- Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Long Name
Thymic Stromal Lymphopoietin Receptor
Alternate Names
CRL2, CRLF2, CRLM-2, PCOR1, TSLP R
Gene Symbol
CRLF2
UniProt
Additional TSLPR Products
Product Documents for Recombinant Human TSLPR Fc Chimera Alexa Fluor® 647 Protein
Product Specific Notices for Recombinant Human TSLPR Fc Chimera Alexa Fluor® 647 Protein
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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