Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI1298

Biotinylated
R&D Systems, part of Bio-Techne
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AVI1298-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q9BZM5.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all ULBP-2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ULBP-2 protein
Human ULBP-2
(Gly26-Ser217)
Accession # Q9BZM5.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly26

Predicted Molecular Mass

50 kDa

SDS-PAGE

60-70 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NKG2D/CD314 Fc Chimera  (Catalog # 1299-NK) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ULBP-2 Fc Chimera Avi-tag (Catalog # AVI1298) binds with an ED50 of 0.500-8.00 ng/mL.

Scientific Data Images for Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human NKG2D/CD314 Fc Chimera Protein (1299-NK) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ULBP‑2 Fc Chimera Avi-tag Protein (Catalog # AVI1298) binds with an ED50 of 0.500-8.00 ng/mL.

Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein (Catalog # AVI1298) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-70 kDa and 120-140 kDa, respectively.

Formulation, Preparation and Storage

AVI1298
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ULBP-2

ULBP-2 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. The name ULBP-2 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-2 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-2 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma delta T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Our Avi-tag Biotinylated Recombinant Human ULBP-2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Cosman, D. et al. (2001) Immunity 14:123.
  2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
  3. Sutherland, C. et al. (2002) J. Immunol. 168:671.
  4. Steinle, A. et al. (2001) Immunogenetics 53:279.
  5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Radosavljevic, M. et al. (2002) Genomics 79:114.
  8. NKG2D and its Ligands (2002) www.rndsystems.com.

Long Name

UL16 Binding Protein-2

Alternate Names

ALCAN-alpha, N2DL2, NKG2DL2, RAET1H, ULBP2

Entrez Gene IDs

80328 (Human)

Gene Symbol

ULBP2

UniProt

Q9BZM5.1

Additional ULBP-2 Products

Product Documents for Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human ULBP-2 Fc Chimera Avi-tag Protein, CF

For research use only

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