Recombinant Human Vanin-1/VNN1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7999-AH

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

O95497

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Vanin-1/VNN1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Vanin-1/VNN1 protein
Gln22-Ser490, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln22 predicted

Predicted Molecular Mass

53 kDa

SDS-PAGE

60-80 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze pantetheine to pantothenate and cysteamine.
The specific activity is >1500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7999-AH
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Vanin-1/VNN1

Vanin‑1 (VNN1) is a member of the biotinidase family and is expressed at the cell surface in epithelial cells (1). VNN1 is also known as vascular non‑inflammatory molecule 1. It does not possess biotinidase activity, but is a pantetheinase that catalyzes the hydrolysis of pantetheine to pantothenic acid (vitamin‑B5) and cysteamine (2, 3). VNN1 is considered to be a potential biomarker for the acute kidney injury (4) and a target for therapeutic intervention in inflammatory bowel disease (5). Recombinant human VNN1 was engineered to have a C‑terminal truncation that prevents the normal GPI-anchor modification, resulting in its secretion.

References

  1. Pitari, G. et al. (2000) FEBS Lett. 483:149.
  2. Maras, B. et al. (1999) FEBS Lett. 461:149.
  3. Martin, F. et al. (2001) Immunogenetics 53:296.
  4. Hosohata, K. et al. (2012) J. Phamacol. Exp. Ther. 341:656.
  5. Berruyer, C. et al. (2006) J. Exp. Med. 203:2817.

Alternate Names

HDLCQ8, Pantetheinase, Pantetheine hydrolase, Tiff66, Vanin1, VNN1

Entrez Gene IDs

8876 (Human); 22361 (Mouse); 29142 (Rat)

Gene Symbol

VNN1

UniProt

O95497

Additional Vanin-1/VNN1 Products

Product Documents for Recombinant Human Vanin-1/VNN1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Vanin-1/VNN1 Protein, CF

For research use only

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