Recombinant Human Versican Isoform V3 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3054-VN

R&D Systems, part of Bio-Techne
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3054-VN-050
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Key Product Details

Source

CHO

Accession #

P13611-4

Conjugate

Unconjugated

Applications

Bioactivity

View all Versican Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Versican protein
Leu21-Tyr646, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu21

Predicted Molecular Mass

71 kDa

SDS-PAGE

78-89 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Versican Isoform V3 (Catalog # 3054-VN) is coated at 0.25 μg/mL, 100 μL/well, Biotinylated Hyalurinan binds with an ED50 of 0.25-1.5 ng/mL.

Scientific Data Images for Recombinant Human Versican Isoform V3 His-tag Protein, CF

Recombinant Human Versican Isoform V3 His-tag Protein Binding Activity

Recombinant Human Versican Isoform V3 His-tag Protein Binding Activity

When Recombinant Human Versican Isoform V3 (Catalog # 3054-VN) is coated at 0.25 μg/mL, 100 μL/well, Biotinylated Hyalurinan binds with an ED50 of 0.25-1.5 ng/mL.
Recombinant Human Versican Isoform V3 His-tag Protein SDS-PAGE

Recombinant Human Versican Isoform V3 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Versican Isoform V3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-89 kDa and 67-80 kDa, respectively.

Formulation, Preparation and Storage

3054-VN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Versican

Versican (versatile proteoglycan; also known as PG-M, CSPG2, ERVR, GHAP, WGN, WGN1) is a 500-600 kDa, secreted member of the large aggregating chondroitin sulfate proteoglycan family, which includes aggrecan, neurocan, and brevican (1-3). The canonical sequence of human Versican (Isoform V0) is 3396 amino acids (aa) in length. It contains an N-terminal hyaluronan-binding region, two large, alternately spliced GAG attachment domains (GAG alpha and GAG beta), two EGF-like domains, a selectin-like region, and a complement regulatory protein-like domain (2). Human Versican has multiple alternative splice variants that include variations mainly in the GAG regions. This product is the Isoform V3 which includes one mutation at aa 348 (Pro to Arg) and lacks of the entire GAG domains (aa 349-3089) compared to Isoform V0. Versican is known to associate with hyaluronan (4), tenascin (5-7), fibronectin (8), fibulin (9), and CD44 (10) in the extracellular matrix that may contribute to the malignant properties of tumor cells (11). It has an anti-adhesive effect on cells, facilitating tumor migration and invasion (12). The multiple alternative splice variants of human Versican could affect cell-cell and cell-matrix adhesion (13-15), migration (16), proliferation, apoptosis (17), and mesenchymal-epithelial transition (18). The Versican gene is expressed in several different tissues, and it is over-expressed in several cancer types, including prostate (19), brain (20), ovary (21), breast (22) and melanoma (11).

References

  1. Shinomura, T. et al. (1993) J. Biol. Chem. 268:14461.
  2. Zimmermann, D. R. and E.Ruoslahti (1989) EMBO J. 8:2975.
  3. Margolis, R. U. and R. K. Margolis (1994) Methods Enzymol. 245:105.
  4. Lebaron, R. G. et al. (1992) J. Biol. Chem. 267:10003.
  5. Perides, G. et al. (1993) Anat. Embryol. (Berl.) 188:467.
  6. Aspberg, A. et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92:10590.
  7. Aspberg, A. et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94:10116.
  8. Braunewell, K. H. et al. (1995) Eur. J. Neurosci. 7:805.
  9. Olin, A. I. et al. (2001) J. Biol. Chem. 276:1253.
  10. Kawashima, H. et al. (2000) J. Biol. Chem. 275:35448.
  11. Touab, M. et al. (2002) Am. J. Pathol. 160:549.
  12. Yamagata, M. et al. (1989) J. Biol. Chem. 264:8012.
  13. Wu, Y. et al. (2002) J. Biol. Chem. 277:12294.
  14. Zheng, P. S. et al. (2004) J. Cell Sci. 117:5887.
  15. Lemire, J. M. et al. (2002) J. Cell Physiol. 190:38.
  16. Mjaatvedt, C.H. et al. (1998) Dev. Biol. 202:56.
  17. Sheng, W. et al. (2005) Mol. Biol. Cell. 16:1330.
  18. Sheng, W. et al. (2006) Mol. Biol. Cell. 17:2009.
  19. Ricciardelli, C. et al. (1998) Clin. Cancer Res. 4:963.
  20. Paulus, W. et al. (1996) J. Neuropathol. Exp. Neurol. 55:528.
  21. Voutilainen, K. et al. (2003) Int. J. Cancer 107:359.
  22. Nara, Y. et al. (1997) Histochem. J. 29:21.

Alternate Names

CSPG2, GHAP, PG-M, VCAN

Entrez Gene IDs

1462 (Human); 13003 (Mouse)

Gene Symbol

VCAN

UniProt

P13611-4

Additional Versican Products

Product Documents for Recombinant Human Versican Isoform V3 His-tag Protein, CF

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Product Specific Notices for Recombinant Human Versican Isoform V3 His-tag Protein, CF

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