Recombinant Human VISTA/B7-H5/PD-1H His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9057-B7

Analyzed by SEC-MALS
R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q9H7M9

Conjugate

Unconjugated

Applications

Bioactivity

View all VISTA/B7-H5/PD-1H Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human VISTA/B7-H5/PD-1H protein
Phe33-Ala194, with an Asp187Glu substitution and a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe33

Predicted Molecular Mass

19 kDa

SDS-PAGE

30-65 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 0.5-3 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 9057-B7 in the following applications:

Scientific Data Images for Recombinant Human VISTA/B7-H5/PD-1H His-tag Protein, CF

Recombinant Human VISTA/B7-H5/PD-1H His-tag Protein SEC-MALS.

Recombinant Human VISTA/B7-H5/PD-1H (Catalog # 9057-B7) has a molecular weight (MW) of 25.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human VISTA/B7-H5/PD-1H His-tag Protein Bioactivity

Recombinant Human VISTA/B7-H5/PD-1H His-tag Protein Bioactivity

Recombinant Human VISTA/B7-H5/PD-1H inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.

Formulation, Preparation and Storage

9057-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VISTA/B7-H5/PD-1H

Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7-H5, is a 55-65 kDa transmembrane glycoprotein with homology to B7-like immune costimulatory molecules (1, 2). Mature human Gi24 contains a 162 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human Gi24 shares 70% and 67% aa sequence identity with mouse and rat Gi24, respectively (3). The 30 kDa ECD can be shed by MT1-MMP, with a 25-30 kDa fragment remaining in the membrane (3). Gi24 promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2 (3). Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC, but is also down-regulated following BMP-4 exposure (4, 5). It binds to BMP-4 directly, and also associates with the type I BMP receptor Activin RIB/ALK-4 (4, 5). Gi24 is expressed on the surface of naïve CD4+ T cells, regulatory T cells, and adipocytes (6, 7). It is up-regulated in vivo on activated monocytes and dendritic cells (5). Gi24 inhibits CD4+ and CD8+ T cell proliferation, and their production of IL-2 and IFN-gamma (6). Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression (6). In contrast, Gi24 limits disease progression in the autoimmune disease model EAE (6). Gi24 also acts as a co-inhibitory receptor on alloreactive T cells to regulate T cell tolerance (8).

References

  1. Flajnik, M.F. et al. (2012) Immunogenetics 64:571.
  2. Wilcox, R.A. et al. (2012) Eur. J. Haematol. 88:465.
  3. Sakr, M.A. et al. (2010) Cancer Sci. 101:2368.
  4. Aloia, L. et al. (2010) J. Biol. Chem. 285:7776.
  5. Parisi, S. et al. (2012) FASEB J. 26:3957.
  6. Wang, L. et al. (2011) J. Exp. Med. 208:577.
  7. Ren, G. et al. (2013) PLoS One 8:e65531.
  8. Flies, D.B. et al. (2015) J. Immunol. 194:5294.

Alternate Names

4632428N05Rik, B7-H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, SISP1, VSIR

Entrez Gene IDs

64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)

Gene Symbol

VSIR

UniProt

Q9H7M9 (Human)

Additional VISTA/B7-H5/PD-1H Products

Product Documents for Recombinant Human VISTA/B7-H5/PD-1H His-tag Protein, CF

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Product Specific Notices for Recombinant Human VISTA/B7-H5/PD-1H His-tag Protein, CF

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