Recombinant Human VSIG10L Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9600-VS

R&D Systems, part of Bio-Techne
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9600-VS-050
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Key Product Details

Source

HEK293

Accession #

Q86VR7-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG10L Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human VSIG10L protein
Human VSIG10L
(Leu28-Ala776)
Accession # Q86VR7-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu28 and Ser176

Predicted Molecular Mass

106 kDa

SDS-PAGE

120-140 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 1-6 μg/mL.

Scientific Data Images for Recombinant Human VSIG10L Fc Chimera Protein, CF

Recombinant Human VSIG10L Fc Chimera Protein Bioactivity

Recombinant Human VSIG10L Fc Chimera Protein Bioactivity

Recombinant Human VSIG10L Fc Chimera (Catalog # 9600-VS) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-6 μg/mL.

Formulation, Preparation and Storage

9600-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG10L

V-set and immunoglobulin domain-containing protein 10-like (VSIG-10L) is an 840 amino acid (aa) single-pass type I membrane protein of the Ig superfamily. The protein consists of a 749 aa extracellular domain (ECD), a 21 aa helical transmembrane domain, and a 70 aa cytoplasmic domain. The ECD of VSIG-10L contains both an Ig-like C2-type 1 domain and an Ig-like C2-type 2 domain. The ECD of mature human VSIG-10L shares 95%, 75%, and 74% identity with Rhesus macaque, mouse, and rat, respectively. The specific biological function of VSIG-10L has yet to be elucidated, but there is evidence to suggest the molecule is involved in cell-cell adhesion. Ig-like C2-type domains are similar to antibody constant domains and can be found in T cell adhesion molecules, such as Cluster of Differentiation 2 (CD2) along with vascular and intercellular cell adhesion molecules (VCAM and ICAM, respectively) (1). VSIG-10L is highly expressed in healthy esophagus with squamous mucosa (2). Down-regulation of the protein may be linked to familial Barrett's esophagus and esophageal adenocarcinoma (2) while up-regulation may be linked to lung squamous cell carcinoma (3). R&D in house data indicate that VSIG-10L inhibits the secretion of IFN-g from anti-CD3 activated human PBMCs.

References

  1. Yang H and Reinherz E (2001) J. Biol. Chem. 276(22):18775.
  2. Fecteau RE, et al. (2016) JAMA Oncol. 2(10):1333.
  3. Tian F, et al. (2017) J. Thorac. Dis. 9(1):42.

Long Name

V-Set and Immunoglobulin Domain Containing 10 Like

Alternate Names

V-Set And Immunoglobulin Domain Containing 10 Like, V-Set And Immunoglobulin Domain-Containing Protein 10-Like

Entrez Gene IDs

147645 (Human); 75690 (Mouse); 308556 (Rat)

Gene Symbol

VSIG10L

UniProt

Q86VR7-1

Additional VSIG10L Products

Product Documents for Recombinant Human VSIG10L Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human VSIG10L Fc Chimera Protein, CF

For research use only

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