Recombinant Human VSIG10L His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9599-VS

R&D Systems, part of Bio-Techne
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9599-VS-050
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Key Product Details

Source

HEK293

Accession #

Q86VR7-1

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG10L Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human VSIG10L protein
Leu28-Ala776, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu28

Predicted Molecular Mass

80 kDa

SDS-PAGE

100-130 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 0.3-1.8 μg/mL.

Scientific Data Images for Recombinant Human VSIG10L His-tag Protein, CF

Recombinant Human VSIG10L His-tag Protein Bioactivity

Recombinant Human VSIG10L His-tag Protein Bioactivity

Recombinant Human VSIG10L (Catalog # 9599-VS) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.3-1.8 μg/mL.

Formulation, Preparation and Storage

9599-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG10L

V-set and immunoglobulin domain-containing protein 10-like (VSIG-10L) is an 840 amino acid (aa) single-pass type I membrane protein of the Ig superfamily. The protein consists of a 749 aa extracellular domain (ECD), a 21 aa helical transmembrane domain, and a 70 aa cytoplasmic domain. The ECD of VSIG-10L contains both an Ig-like C2-type 1 domain and an Ig-like C2-type 2 domain. The ECD of mature human VSIG-10L shares 95%, 75%, and 74% identity with Rhesus macaque, mouse, and rat, respectively. The specific biological function of VSIG-10L has yet to be elucidated, but there is evidence to suggest the molecule is involved in cell-cell adhesion. Ig-like C2-type domains are similar to antibody constant domains and can be found in T cell adhesion molecules, such as Cluster of Differentiation 2 (CD2) along with vascular and intercellular cell adhesion molecules (VCAM and ICAM, respectively) (1). VSIG-10L is highly expressed in healthy esophagus with squamous mucosa (2). Down-regulation of the protein may be linked to familial Barrett's esophagus and esophageal adenocarcinoma (2) while up-regulation may be linked to lung squamous cell carcinoma (3). R&D in house data indicate that VSIG-10L inhibits the secretion of IFN-g from anti-CD3 activated human PBMCs.

References

  1. Yang H and Reinherz E (2001) J. Biol. Chem. 276(22):18775.
  2. Fecteau RE, et al. (2016) JAMA Oncol. 2(10):1333.
  3. Tian F, et al. (2017) J. Thorac. Dis. 9(1):42.

Long Name

V-Set and Immunoglobulin Domain Containing 10 Like

Alternate Names

V-Set And Immunoglobulin Domain Containing 10 Like, V-Set And Immunoglobulin Domain-Containing Protein 10-Like

Entrez Gene IDs

147645 (Human); 75690 (Mouse); 308556 (Rat)

Gene Symbol

VSIG10L

UniProt

Q86VR7-1

Additional VSIG10L Products

Product Documents for Recombinant Human VSIG10L His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human VSIG10L His-tag Protein, CF

For research use only

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