Recombinant Human VSIG8 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9200-VS

R&D Systems, part of Bio-Techne
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9200-VS-050
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Key Product Details

Source

CHO

Accession #

Q5VU13

Structure / Form

Disulfide-linked homodimer 

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG8 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VSIG8 protein
Human VSIG8
(Val22-Gly263)
Accession # Q5VU13		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val22

Predicted Molecular Mass

54 kDa

SDS-PAGE

56-64 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 1-5 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 9200-VS in the following applications:

Scientific Data Images for Recombinant Human VSIG8 Fc Chimera Protein, CF

Recombinant Human VSIG8 Fc Chimera Protein Bioactivity

Recombinant Human VSIG8 Fc Chimera Protein Bioactivity

Recombinant Human VSIG8 Fc Chimera (Catalog # 9200-VS) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-5 μg/mL.

Formulation, Preparation and Storage

9200-VS
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, Mannitol and Trehalose.
Reconstitution
Reconstitute at 200 μg/mL in Water.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature human VSIG8 consists of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, human VSIG8 shares 88% and 89% aa identity with mouse and rat VSIG8, respectively. Alternative splicing generates a long isoform of human VSIG8 with a substitution in the cytoplasmic juxtamembrane region and a 124 aa extension at the C-terminus. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References

  1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
  2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
  3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.

Long Name

V-Set and Immunoglobulin Domain Containing 8

Alternate Names

V-set and immunoglobulin domain containing 8

Entrez Gene IDs

391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)

Gene Symbol

VSIG8

UniProt

Q5VU13

Additional VSIG8 Products

Product Documents for Recombinant Human VSIG8 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human VSIG8 Fc Chimera Protein, CF

For research use only

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