Recombinant Mouse ADAM10 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 946-AD
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Structure / Form
Recombinant Mouse ADAM10 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag.
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse ADAM10 protein
Thr215-Glu673, with a C-terminal 10-His tag
Thr215-Glu673, with a C-terminal 10-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr215
Predicted Molecular Mass
52 kDa
SDS-PAGE
60 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003).
The specific activity is >5 pmol/min/µg, as measured under the described conditions.
The specific activity is >5 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
946-AD
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES, NaCl, CaCl2, ZnCl2, and Brij-35. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM Tris, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ADAM10
ADAM10 (also known as Kuzbanian, mammalian disintegrin metalloprotease, myelin-associated metalloproteinase) is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1, 2). Like other membrane-anchored ADAMs, ADAM10 consists of the following domains, pro with a cysteine switch and furin cleavage sequence, catalytic with the zinc-binding site and Met-turn expected for reprolysins, disintegrin-like, cysteine-rich, EGF-like, transmembrane, and cytoplasmic. ADAM10 is highly conserved, with 97% amino acid identity between mouse, rat, bovine and human and 45% identity between mouse and Drosophila. The active enzyme processes notch, notch ligand delta, and amyloid protein precursor at the alpha site, playing an important role in neurogenesis (3, 4). It also processes the 26 kDa membrane-anchored pro-tumor necrosis factor-alpha (TNF-alpha) to the 17 kDa mature TNF-alpha (5). It cleaves myelin basic protein and type IV collagen (6, 7). ADAM10 is widely expressed in tissues and resides both on the cell surface and in the cell (8, 9).
References
- Rooke, J. et al. (1996) Science 273:1227.
- Pan, D. and Rubin, G.M. (1997) Cell 90:271.
- Qi, H. et al. (1999) Science 283:91.
- Lammich, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3922.
- Rosendahl, M.S. et al. (1997) J. Biol. Chem. 272:24588.
- Chantry, A. et al. (1989) J. Biol. Chem. 264:21603.
- Millichip, M.I. et al. (1998) Biochem. Biophys. Res. Comm. 245:594.
- Chantry, A. and Glynn, P. (1990) Biochem. J. 268:245.
- Fahrenholz, F.S. et al. (2000) Ann. N.Y. Acad. Sci. 920:215.
Long Name
A Disintegrin and Metalloprotease-like Domain 10
Alternate Names
CD156c, Kuzbanian, MADM
Gene Symbol
ADAM10
UniProt
Additional ADAM10 Products
Product Documents for Recombinant Mouse ADAM10 Protein, CF
Product Specific Notices for Recombinant Mouse ADAM10 Protein, CF
For research use only
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