Recombinant Mouse AMIGO3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9425-AM

R&D Systems, part of Bio-Techne
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9425-AM-050
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Key Product Details

Source

NS0

Accession #

Q8C2S7

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all AMIGO3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse AMIGO3 protein
Mouse AMIGO3
(Thr20-Pro378)
Accession # Q8C2S7		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr20

Predicted Molecular Mass

67 kDa

SDS-PAGE

78-91 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 0.3-1.5 μg/mL.

Scientific Data Images for Recombinant Mouse AMIGO3 Fc Chimera Protein, CF

Recombinant Mouse AMIGO3 Fc Chimera Protein Bioactivity

Recombinant Mouse AMIGO3 Fc Chimera Protein Bioactivity

Recombinant Mouse AMIGO3 Fc Chimera (Catalog # 9425-AM) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.3-1.5 μg/mL.

Formulation, Preparation and Storage

9425-AM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMIGO3

Amphoterin-Induced Gene and ORF 3 (AMIGO3), also known as Alvin-3, is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine‑rich repeats (LRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied (1). Mouse AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and AMIGO2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with both that of rat and human AMIGO3, respectively. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties and are thought to play roles in neuronal axon tract development and cell adhesion. AMIGO3 mRNA and protein levels are preferentially and significantly raised in DRGN and RGC immediately after central axotomy. Depression of AMIGO3 expression correlates with dorsal column (DC) and optic nerve regeneration. AMIGO3 interacts with NgR1 and p75/TROY forming a functional receptor complex that activates RhoGTP in cells exposed to CNS myelin extracts (CME). AMIGO3 substitutes for LINGO-1 in centrally axotomized DRGN and RGC in the acute phase of injury. AMIGO3-NgR1-p75/TROY receptor complex mediates immediate axon growth inhibitory responses to CNS myelin (2). Our in-house data showed that AMIGO-3 inhibited anti-CD3 induced IL-2 secretion on CD3+ cells, suggesting that AMIGO-3 may be involved in T cell activation.

References

  1. Kuja-Panula J, et al. (2003). J Cell Biol 160:963.
  2. Ahmed Z, et al. (2013) PLoS One. 16:e61878.

Long Name

Amphoterin-induced Gene and ORF 3

Alternate Names

adhesion molecule with Ig-like domain 3, ALI3, alivin-3, AMIGO-3, amphoterin-induced gene and ORF 3, amphoterin-induced protein 3, KIAA1851, MGC120552

Entrez Gene IDs

386724 (Human); 320844 (Mouse)

Gene Symbol

AMIGO3

UniProt

Q8C2S7

Additional AMIGO3 Products

Product Documents for Recombinant Mouse AMIGO3 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse AMIGO3 Fc Chimera Protein, CF

For research use only

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