Recombinant Mouse Aminoacylase/ACY1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2700-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Aminoacylase/ACY1 protein
Met1-Ser408, with a C-terminal 10-His tag
Accession # NP_079647
Met1-Ser408, with a C-terminal 10-His tag
Accession # NP_079647
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln18 predicted
Predicted Molecular Mass
45 kDa
SDS-PAGE
42 kDa, reducing conditions
Activity
Measured by its ability to cleave N-acetyl-L-Methione (Ac-Met).
The specific activity is >4,000 pmol/min/µg, as measured under the described protocol.
The specific activity is >4,000 pmol/min/µg, as measured under the described protocol.
Formulation, Preparation and Storage
2700-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Aminoacylase/ACY1
The mouse ACY1 gene encodes aminoacylase, a member of the M20 family of metalloproteases (1). ACY1 plays a general role in the cytosolic breakdown of acetylated amino acids generated during protein degradation. It also interacts with sphingosine kinase type 1, which is involved in promoting cell growth and inhibiting apoptosis of tumor cells (2). The full-length protein is expressed and the purified enzyme is active in the assay described in the Activity Assay Protocol.
References
- Lindner, H.A. et al. (2003) J. Biol. Chem. 278:44496.
- Maceyka, M. et al. (2004) FEBS Lett. 568:30.
Alternate Names
ACY1
Gene Symbol
ACY1
UniProt
Additional Aminoacylase/ACY1 Products
Product Documents for Recombinant Mouse Aminoacylase/ACY1 Protein, CF
Product Specific Notices for Recombinant Mouse Aminoacylase/ACY1 Protein, CF
For research use only
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