Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9983-AN

R&D Systems, part of Bio-Techne
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9983-AN-050
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Key Product Details

Source

NS0

Accession #

Q8R1L8

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Angiopoietin-like Protein 8/Betatrophin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Angiopoietin-like Protein 8/Betatrophin protein
Mouse Betatrophin
(Ala22-Ala198)
Accession # Q8R1L8		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

47 kDa

SDS-PAGE

52-56 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Angiopoietin-like 3 (Catalog # 9899-AN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse Angiopoietin-like Protein 8/Betatrophin Fc Chimera binds with an ED50 of 0.12-0.72 μg/mL.

Scientific Data Images for Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein, CF

Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein Binding Activity

Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein Binding Activity

When Recombinant Mouse Angiopoietin-like 3 (Catalog # 9899-AN) is immobilized at 1 µg/mL, Recombinant Mouse Betatrophin Fc Chimera (Catalog # 9983-AN) binds with an ED50 of 0.12-0.72 µg/mL.
Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein SDS-PAGE

Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Angiopoietin-like Protein 8/Betatrophin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-56 kDa and 105-110 kDa, respectively.

Formulation, Preparation and Storage

9983-AN
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS,NaCl and CHAPS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Angiopoietin-like Protein 8/Betatrophin

Betatrophin (also called ANGPTL8, lipasin and RIPL), a novel secretory protein from liver and fatty tissues, is believed to be involved in lipid and glucose metabolism. It is most homologous to ANGPTL3 but lacks the fibrinogen-like domain of ANGPTL3 and other ANGPTL family members (1). Mouse Betatrophin is 183 amino acids (aa) in length and shares 72% aa identity with human Betatrophin. Betatrophin is a crucial modulator in lipid metabolism. It can form a complex with the N-terminal of ANGPTL3, and the complex is necessary for inhibition of LPL and triglyceride modulation (2). Betatrophin did not control beta-cell expansion in the mouse model (3). In addition to the lipid and glucose metabolism, ANGPTL8 has been reported to be involved in many other disorders (4).

References

  1. Quagliarini, F. et al. (2012) PNAS 109(48):19751.
  2. Chi, X., et al. (2017) Molecular Metabolism 6:1137.
  3. Cox A.R.  et al. PLoS One (2016) 11:e0159276.
  4. Luo, M. et al. (2018) Front Endocrino 9:169.

Alternate Names

Angiopoietin like Protein 8, ANGPTL8, Betatrophin, C19orf80, Gm6484, Lipasin, PRO1185, PVPA599, RIFL, TD26

Entrez Gene IDs

55908 (Human); 624219 (Mouse); 100361444 (Rat)

Gene Symbol

ANGPTL8

UniProt

Q8R1L8

Additional Angiopoietin-like Protein 8/Betatrophin Products

Product Documents for Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein, CF

For research use only

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