Recombinant Mouse Arylsulfatase A/ARSA Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2858-SU
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Arylsulfatase A/ARSA protein
Ser18-Ser506, with a C-terminal 10-His tag
Ser18-Ser506, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser18
Predicted Molecular Mass
54 kDa
SDS-PAGE
60 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
The specific activity is >100 pmol/min/µg, as measured under the described conditions.
The specific activity is >100 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2858-SU
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Arylsulfatase A/ARSA
As a member of the sulfatase family, arylsulfatase A is encoded by the ARSA gene and required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet (1). ARSA deficiency results in metachromatic leukodystrophy (MLD), a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms (2). The deduced amino acid sequence of mouse ARSA consists of a signal peptide (residues 1-17) and a mature chain (residues 18-506) (3). Recombinant mouse ARSA corresponds to the mature chain and has sulfatase activity as described in Activity Assay Protocol.
References
- Lukatela, G. et al. (1998) Biochemistry 37:3654.
- Parenti, G. et al. J. (1997) Curr. Opin. Genet. & Dev. 7:386.
- Kreysing, J. et al. (1994) Genomics 19:249.
Alternate Names
ARSA, Cerebroside 3-sulfatase
Gene Symbol
ARSA
UniProt
Additional Arylsulfatase A/ARSA Products
Product Documents for Recombinant Mouse Arylsulfatase A/ARSA Protein, CF
Product Specific Notices for Recombinant Mouse Arylsulfatase A/ARSA Protein, CF
For research use only
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