Recombinant Mouse BCAM Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7390-BC

R&D Systems, part of Bio-Techne
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7390-BC-050
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Key Product Details

Source

NS0

Accession #

Q9R069

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BCAM Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BCAM protein
Mouse BCAM
(Met1-Ala541)
Accession # Q9R069		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu26

Predicted Molecular Mass

84 kDa (monomer)

SDS-PAGE

95‑105 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of TE-85 human osteogenic sarcoma cells.
When 5 x 104 cells per well are added to Recombinant Mouse BCAM Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 20‑100 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

7390-BC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAM

Mouse Basal Cell Adhesion Molecule (BCAM), also known as CD239, is a member of immunoglobulin superfamily protein. In human, BCAM is the short splice form of the Lutheran blood group glycoprotein (Lu). Mature mouse BCAM consists of a 516 amino acid (aa) extracellular domain (ECD) with two Ig-like V-type domains and three Ig-like C2-type domains, a 21 aa transmembrane segment, and a 60 aa cytoplasmic domain (1). Within the ECD, mouse BCAM shares 73% and 91% aa sequence identity with human and rat BCAM, respectively. BCAM is widely expressed in epithelial and endothelial tissues including in the vasculature, kidney glomerulus, small intestine, colon, hair follicle outer root sheath, and basal keratinocytes of the skin during inflammation (2‑5). BCAM is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle (2, 4, 6, 7). BCAM is up‑regulated on carcinomas (particularly ovarian), sarcomas, astrocytomas, and melanomas (2, 3, 5, 6). It cooperates with Integrins beta1 and alphaV beta3 as an adhesion receptor for Laminins which contain the alpha5 chain (8, 9). Mouse BCAM binds to both human and mouse Laminin, whereas human BCAM binds to human but not to mouse Laminin (1). In contrast to mouse, human BCAM is additionally expressed on erythrocytes and is up‑regulated on these cells in sickle cell disease and polycythemia vera (4, 10, 11). In human erythroid disorders, it mediates increased binding of erythrocytes to Laminin and promotes the formation of erythrocyte-monocyte aggregates (4, 10‑14).

References

  1. Rahuel, C. et al. (1999) Immunogenetics 50:271.
  2. Garin-Chesa, P. et al. (1994) Int. J. Oncol. 5:1261.
  3. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.
  4. Rahuel, C. et al. (2008) Am. J. Physiol. Renal Physiol. 294:F393.
  5. Rettig, W.J. et al. (1986) Cancer Res. 46:6406.
  6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. 85:3110.
  7. Nishimune, H. et al. (2008) J. Cell Biol. 182:1201.
  8. Kikkawa, Y. et al. (2007) J. Biol. Chem. 282:14853.
  9. Vainionpaa, N. et al. (2006) Am. J. Physiol. Cell. Physiol. 290:C764.
  10. Zen, Q. et al. (1999) J. Biol. Chem. 274:728.
  11. Wautier, M.-P. et al. (2007) Blood 110:894.
  12. Udani, M. et al. (1998) J. Clin. Invest. 101:2550.
  13. Chaar, V. et al. (2010) Haematologica 95:1841.
  14. Gauthier, E. et al. (2009) Br. J. Haematol. 148:456.

Long Name

Basal Cell Adhesion Molecule

Alternate Names

CD239

Entrez Gene IDs

4059 (Human); 57278 (Mouse)

Gene Symbol

BCAM

UniProt

Q9R069

Additional BCAM Products

Product Documents for Recombinant Mouse BCAM Fc Chimera Protein, CF

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