Recombinant Mouse BCAM His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11174-BC

R&D Systems, part of Bio-Techne
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11174-BC-050
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Key Product Details

Source

NS0

Accession #

Q9R069.1

Conjugate

Unconjugated

Applications

Bioactivity

View all BCAM Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BCAM protein
Glu26-Ala541, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu26

Predicted Molecular Mass

58 kDa

SDS-PAGE

65-85 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of TE-85 human osteogenic sarcoma cells.
The ED50 for this effect is 18.0-162 ng/mL.

Scientific Data Images for Recombinant Mouse BCAM His-tag Protein, CF

Recombinant Mouse BCAM His-tag Protein Bioactivity.

Recombinant Mouse BCAM His-tag Protein (Catalog # 11174-BC) supports the adhesion of TE‑85 human osteogenic sarcoma cells. The ED50 for this effect is 18.0-162 ng/mL.

Recombinant Mouse BCAM His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse BCAM His-tag Protein (Catalog # 11174-BC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-85 kDa.

Formulation, Preparation and Storage

11174-BC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAM

Basal Cell Adhesion Molecule (BCAM), also known as CD239, is a member of immunoglobulin superfamily protein. BCAM arises from alternate splicing of the Lutheran blood group molecule (Lu) and lacks a 40 amino acid (aa) SH3-containing segment that is present in the cytoplasmic domain of Lutheran (1). Mature mouse BCAM consists of two Ig-like V-type domains and three Ig-like C2-type domains in the extracellular domain (ECD), a transmembrane segment, and a short cytoplasmic domain. Within the ECD, mouse BCAM shares 73% and 91% aa sequence identity with human and rat BCAM, respectively. BCAM is widely expressed in epithelial and endothelial tissues including in the vasculature, kidney glomerulus, small intestine, colon, hair follicle outer root sheath, and basal keratinocytes of the skin during inflammation (2‑5). BCAM is also expressed on vascular and visceral smooth muscle cells and at the neuromuscular junction of skeletal muscle (2, 4, 6, 7). It cooperates with Integrins beta 1 and alpha V beta 3 as an adhesion receptor for Laminins which contain the alpha 5 chain (8, 9). Mouse BCAM binds to both human and mouse Laminin, whereas human BCAM binds to human but not to mouse Laminin (1). BCAM is up‑regulated on carcinomas (particularly ovarian), sarcomas, astrocytomas, and melanomas (2, 3, 5, 6). In contrast to mouse, human BCAM is additionally expressed on erythrocytes and is up‑regulated on these cells in sickle cell disease and polycythemia vera (4, 10, 11). In human erythroid disorders, it mediates increased binding of erythrocytes to Laminin and promotes the formation of erythrocyte-monocyte aggregates (4, 10‑14).

References

  1. Rahuel, C. et al. (1999) Immunogenetics 50:271.
  2. Garin-Chesa, P. et al. (1994) Int. J. Oncol. 5:1261.
  3. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.
  4. Rahuel, C. et al. (2008) Am. J. Physiol. Renal Physiol. 294:F393.
  5. Rettig, W.J. et al. (1986) Cancer Res. 46:6406.
  6. Rettig, W.J. et al. (1988) Proc. Natl. Acad. Sci. 85:3110.
  7. Nishimune, H. et al. (2008) J. Cell Biol. 182:1201.
  8. Kikkawa, Y. et al. (2007) J. Biol. Chem. 282:14853.
  9. Vainionpaa, N. et al. (2006) Am. J. Physiol. Cell. Physiol. 290:C764.
  10. Zen, Q. et al. (1999) J. Biol. Chem. 274:728.
  11. Wautier, M.-P. et al. (2007) Blood 110:894.
  12. Udani, M. et al. (1998) J. Clin. Invest. 101:2550.
  13. Chaar, V. et al. (2010) Haematologica 95:1841.
  14. Gauthier, E. et al. (2009) Br. J. Haematol. 148:456.

Long Name

Basal Cell Adhesion Molecule

Alternate Names

CD239

Entrez Gene IDs

4059 (Human); 57278 (Mouse)

Gene Symbol

BCAM

UniProt

Q9R069.1

Additional BCAM Products

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Product Specific Notices for Recombinant Mouse BCAM His-tag Protein, CF

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