Recombinant Mouse beta-NGF Protein
R&D Systems, part of Bio-Techne | Catalog # 1156-NG
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse beta-NGF protein
Ser122-Gly241
Ser122-Gly241
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser122
Predicted Molecular Mass
13.5 kDa
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.1-1 ng/mL.
The ED50 for this effect is 0.1-1 ng/mL.
Reviewed Applications
Read 1 review rated 5 using 1156-NG in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1156-NG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1156-NG/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: beta-NGF
NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system (1). In addition, beta-NGF can act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons (2). beta-NGF has also been shown to have biological effects on a variety of neurons, glia, and nonneural cells (3). NGF was initially isolated from the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta-NGF, exhibits all the biological activities ascribed to NGF (4 - 5). Recombinant mouse beta-NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta-NGF and 95.8% with rat beta-NGF (6).
NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NT-6 and NT-7 were also identified in fish (7, 8). Biological activities of the NGF family are mediated through binding and activation of two types of receptors, TrKs (TrKA, TrKB, and TrKC) and NGF receptor. TrKs are single-pass membrane proteins belonging to the receptor tyrosine kinase family. The neurotrophins bind to TrKs specifically. NGF is specific for TrKA, BDNF and NT-4 for TrKB, and NT-3 mostly for TrKC. NGF receptor is a TNF receptor superfamily protein that binds to all the neurotrophins with similar affinity (9). TrkA is often referred to as the high-affinity receptor. However, most high affinity binding is mediated by the interaction of NGF receptor with TrKA. Both NGF and its receptors are expressed during development, adult life and aging in many cell types in CNS and PNS, immune and inflammatory systems. NGF signaling has been shown to play an important role in neuroprotection and repair (3, 10).
References
- Levi-Montalcini, R and V. Hamberger (1951) J. Exp. Zool. 116:321.
- Levi-Montacini, R. Ann. Rev. Neurosci. 5:341.
- Sofroniew, M.V. et al. (2001) Ann Rev Neurosci. 24:1217.
- Edwards, R.H. et al. (1988) J Biol Chem. 263:6810.
- Angeletti, R H. et al. (1973) Biochemistry 12:100.
- Ullrich, A. et al. (1983) Nature 303:821.
- Gotz, R. et al. (1994) Nature 372:266.
- Lai, K.O. et al. (1998) Mol. Cell. Neurosci. 11:64.
- Bothwell, M. (1995) Ann Rev Neurosci. 18:223.
- E.J. Huang and L.F. Reichardt (2001) Ann Rev Neurosci. 24:677.
Long Name
beta Nerve Growth Factor
Alternate Names
betaNGF, NGFB
Gene Symbol
NGF
UniProt
Additional beta-NGF Products
Product Documents for Recombinant Mouse beta-NGF Protein
Product Specific Notices for Recombinant Mouse beta-NGF Protein
For research use only
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