Recombinant Mouse BMP-10 Protein

R&D Systems, part of Bio-Techne | Catalog # 6038-BP

R&D Systems, part of Bio-Techne
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Carrier Free
6038-BP-025/CF
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With Carrier
6038-BP-025
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Key Product Details

Source

CHO

Accession #

Q9R229

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all BMP-10 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse BMP-10 protein
Asn314-Arg421

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn314

Predicted Molecular Mass

12.1 kDa (monomer)

SDS-PAGE

10 kDa, reducing conditions

Activity

Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.
The ED50 for this effect is 6-36 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 6038-BP
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Carrier Free: 6038-BP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: BMP-10

Bone morphogenetic protein 10 (BMP-10), along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors (1-3). Proteolytic removal of the propeptide from the 60 kDa proprotein yields a 12 kDa mature BMP-10 which forms disulfide-linked nonglycosylated homodimers (4, 5). In transfectants, BMP-10 can also be secreted as a proprotein which binds to matrix fibrillin (4, 6). Mature mouse BMP-10 shares 98% and 100% amino acid sequence identity with human and rat BMP-10, respectively, and 48%-64% with mouse BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart and first appears at the onset of trabeculation and chamber formation (7-9). Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development (8). BMP-10 is required for maintaining expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and promoting the Notch-dependent growth of embryonic cardiomyocytes (8, 10-12). NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes (11). BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size and is upregulated in hypertrophied ventricles (9, 13). Mature BMP-10 accumulates within cardiomyocytes in association with the sarcomere protein Tcap (13). BMP-10 induces signaling through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines (4, 5). Deletion of BMPR-IA or BMP-10 causes similar cardiac morphogenetic abnormalities (14). In dermal endothelial cells, BMP-10 induces migration, proliferation, and gene expression typically associated with ALK-1 (5).

References

  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
  3. Schneider, M.D. et al. (2003) Cytokine Growth Factor Rev. 14:1.
  4. Mazerbourg, S. et al. (2005) J. Biol. Chem. 280:32122.
  5. David, L. et al. (2007) Blood 109:1953.
  6. Sengle, G. et al. (2008) J. Biol. Chem. 283:13874.
  7. Neuhaus, H. et al. (1999) Mech. Dev. 80:181.
  8. Chen, H. et al. (2004) Development 131:2219.
  9. Chen, H. et al. (2006) J. Biol. Chem. 281:27481.
  10. Srivastava, D. and Olson, E.N. (2000) Nature 407:221.
  11. Pashmforoush, M. et al. (2004) Cell 117:373.
  12. Grego-Bessa, J. et al. (2007) Dev. Cell 12:415.
  13. Nakano, N. et al. (2007) Am. J. Heart Circ. Physiol. 293:H3396.
  14. Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.

Long Name

Bone Morphogenetic Protein 10

Alternate Names

BMP10

Entrez Gene IDs

27302 (Human); 12154 (Mouse)

Gene Symbol

BMP10

UniProt

Q9R229

Additional BMP-10 Products

Product Documents for Recombinant Mouse BMP-10 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 6038-BP (with carrier)
Download SDS 6038-BP/CF (carrier free)

Product Specific Notices for Recombinant Mouse BMP-10 Protein

For research use only

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