Recombinant Mouse BTNL4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9594-BT

R&D Systems, part of Bio-Techne
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9594-BT-050
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Key Product Details

Source

NS0

Accession #

NP_109671

Conjugate

Unconjugated

Applications

Bioactivity

View all BTNL4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse BTNL4 protein
Gln29-Trp250, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln29 inferred from enzymatic pyroglutamate treatment revealing Glu30

Predicted Molecular Mass

26 kDa

SDS-PAGE

34-39 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for this effect is 0.8-4 μg/mL.

Scientific Data Images for Recombinant Mouse BTNL4 His-tag Protein, CF

Recombinant Mouse BTNL4 His-tag Protein Bioactivity

Recombinant Mouse BTNL4 His-tag Protein Bioactivity

Recombinant Mouse BTNL4 (Catalog # 9594-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.8-4 μg/mL.

Formulation, Preparation and Storage

9594-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTNL4

Butyrophilin-like 4, also known as BTLN4, BTN3A3 (human) and BTN3A2 (rat) is an approximately 65 kDa member of the butyrophilin family that may play a role in immune regulation (1-3). The mouse BTNL4 is a 586 aa type 1 transmembrane protein, consisting of a 28 aa signal peptide, followed by a 109 aa immunoglobulin (Ig) V domain, a 71 aa IgC domain, a 20 aa transmembrane domain, and a 170 aa cytoplasmic B30.2/SPRY domain (4). Within the extracellular domain including aa 29‑250, mouse BTNL4 shares 45% and 82% sequence identity with human BTN3A3 and rat BTN3A2, respectively. BTNL4 shows striking sequence similarity to Skint1; is also largely restricted to an epithelial tissue (the small intestine) replete with T cells (4). Our in-house studies showed BTNL4 co-inhibited anti-CD3 induced IL‑2 secretion on CD3+ cells.

References

  1. Yamashiro, H. et al. (2010) J. Leukoc Biol. 88:757.
  2. Compte, E. et al. (2004) Eur. J. Immunol. 34:2089.
  3. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  4. Bas, A. et al. (2011) Proc Natl Acad Sci U S A. 108:4376.

Long Name

Butyrophilin-like 4

Alternate Names

BTN3A3, EG632126, NG11

Entrez Gene IDs

632126 (Mouse)

Gene Symbol

BTNL4

UniProt

NP_109671

Additional BTNL4 Products

Product Documents for Recombinant Mouse BTNL4 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse BTNL4 His-tag Protein, CF

For research use only

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