Recombinant Mouse C1qR1/CD93 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1696-CD

R&D Systems, part of Bio-Techne
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1696-CD-050
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Key Product Details

Source

NS0

Accession #

O89103

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all C1qR1/CD93 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse C1q R1/CD93 protein
Mouse C1q R1
(Ala23-Asn572)
Accession # O89103		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23

Predicted Molecular Mass

85.7 kDa (monomer)

SDS-PAGE

120-130 kDa, reducing conditions

Activity

Measured by its ability to induce TNF-alpha secretion by THP-1 human acute monocytic leukemia cells.
The ED50 for this effect is 6-30 μg/mL.

Formulation, Preparation and Storage

1696-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1qR1/CD93

C1q R1, also known as CD93, C1qRp, and AA4, is an approximately 125 kDa transmembrane glycoprotein that is involved in various aspects of inflammatory reactions (1). Mature mouse CD93 consists of a 550 amino acid (aa) extracellular domain (ECD) that contains one C‑type lectin domain, five tandem EGF‑like repeats, and a mucin‑like region. This is followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (2, 3). Within the ECD, mouse CD93 shares 66% and 87% aa sequence identity with human and rat CD93, respectively. C1qR1/CD93 should not be confused with 60 kDa Calreticulin which is also known as C1q R1 (5, 6). Unlike Calreticulin, and despite its name, C1q R1/CD93 does not appear to bind the complement protein C1q (4, 6). CD93 is expressed by vascular endothelial cells (6) and by a variety of hematopoietic cells (4‑10). Various sized fragments of soluble CD93 (50‑75 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a truncated form of the molecule embedded in the cell membrane (12‑14). Cross‑linking of cell surface CD93 enhances phagocytosis by monocytes, and enhances the uptake of apoptotic cells in vivo (11, 16). Soluble CD93 promotes the differentiation of monocytes to macrophages, the phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands (13, 15). It is required for plasma cell longevity in the bone marrow, resulting in an extended humoral response (10).

References

  1. Greenlee-Wacker, M.C. et al. (2012) Curr. Drug Targets 13:411.
  2. Petrenko, O. et al. (1999) Immunity 10:691.
  3. Norsworthy, P.J. et al. (1999) Mamm. Genome 10:789.
  4. Steinberger, P. et al. (2002) J. Leukoc. Biol. 71:133.
  5. Nepomuceno, R.R. and A.J. Tenner (1998) J. Immunol. 160:1929.
  6. McGreal, E.P. et al. (2002) J. Immunol. 168:5222.
  7. Lovik, G. et al. (2001) Scand. J. Immunol. 53:410.
  8. Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441.
  9. Ikewaki, N. et al. (2010) J. Clin. Immunol. 30:723.
  10. Chevrier, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3895.
  11. Norsworthy, P.J. et al. (2004) J. Immunol. 172:3406.
  12. Bohlson, S.S. et al. (2005) J. Immunol. 175:1239.
  13. Greenlee, M.C. et al. (2009) Inflamm. Res. 58:909.
  14. Greenlee-Wacker, M.C. et al. (2011) J. Immunol. 187:3353.
  15. Jeon, J.W. et al. (2010) J. Immunol. 185:4921.
  16. Nepomuceno, R.R. et al. (1999) J. Immunol. 162:3583.

Long Name

Complement Component C1q Receptor

Alternate Names

AA4 Antigen, C1q R1, C1q Rp, C1qRp, CD93, Collectin Receptor, Ly68

Entrez Gene IDs

22918 (Human); 17064 (Mouse); 84398 (Rat)

Gene Symbol

CD93

UniProt

O89103

Additional C1qR1/CD93 Products

Product Documents for Recombinant Mouse C1qR1/CD93 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse C1qR1/CD93 Fc Chimera Protein, CF

For research use only

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