Recombinant Mouse Cadherin-11 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6627-CA

R&D Systems, part of Bio-Techne
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6627-CA-050
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Key Product Details

Source

NS0

Accession #

P55288

Conjugate

Unconjugated

Applications

Bioactivity

View all Cadherin-11 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Cadherin-11 protein
Mouse Cadherin-11
(Met1 - Thr617)
Accession # P55288		 IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe23, Leu25, & Gly54

Predicted Molecular Mass

89.1 kDa (mature protein, monomer); 92.8 kDa (pro-protein, monomer)

SDS-PAGE

100-110 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of CCRT cotton rat osteosarcoma cells.
The ED50 for this effect is 0.35-1.4 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

6627-CA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-11

Cadherin-11, also known as OB-Cadherin, is a 120 kDa member of the classical Cadherin family of calcium-dependent homophilic adhesion proteins. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems (2 ‑ 5). It is expressed on osteoblasts in the adult where it promotes the differentiation of both osteoblasts and chondrocytes (6). Cadherin-11 is up‑regulated on breast cancer and prostate cancer cells which preferentially metastasize to bone (7, 8). It facilitates this metastasis via homophilic adhesion to bone marrow stroma and osteoblast-expressed Cadherin-11 (7 ‑ 9). In the synovium, Cadherin-11 supports adhesion between synoviocytes but promotes cell invasion in synovitis and rheumatoid arthritis (10, 11). Its up‑regulation in the vasculature following injury contributes to intimal hyperplasia by inducing smooth muscle cell migration and proliferation (12). In the nervous system, Cadherin-11 interacts with FGF R1 to promote neurite extension from spinal cord explants (13). Mature mouse Cadherin-11 consists of a 564 amino acid (aa) extracellular domain (ECD) with five tandem Cadherin repeats, a 23 aa transmembrane segment, and a 156 aa cytoplasmic domain (2, 3, 14). Within the ECD, mouse Cadherin-11 shares 97% and 98% aa sequence identity with human and rat Cadherin-11, respectively. An 80 kDa portion of the Cadherin-11 ECD can be shed by proteolytic cleavage, and this fragment competes with the full length molecule for cell adhesion (4, 15).

References

  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Kimura, Y. et al. (1995) Dev. Biol. 169:347.
  3. Hoffmann, I. and R. Balling (1995) Dev. Biol. 169:337.
  4. McCusker, C. et al. (2009) Mol. Biol. Cell 20:78.
  5. Clendenon, S.G. et al. (2009) Dev. Dyn. 238:1909.
  6. Kii, I. et al. (2004) J. Bone Mineral Res. 19:1840.
  7. Tamura, D. et al. (2008) Int. J. Oncol. 33:17.
  8. Chu, K. et al. (2008) Mol. Cancer Res. 6:1259.
  9. Huang, C.-F. et al. (2010) Cancer Res. 70:4580.
  10. Valencia, X. et al. (2004) J. Exp. Med. 200:1673.
  11. Kiener, H.P. et al. (2009) Arthritis Rheum. 60:1305.
  12. Monahan, T.S. et al. (2007) J. Vasc. Surg. 45:581.
  13. Boscher, C. and R.-M. Mege (2008) Cell. Signal. 20:1061.
  14. Okazaki, M. et al. (1994) J. Biol. Chem. 269:12092.
  15. Kawaguchi, J. et al. (1999) J. Bone Mineral Res. 14:764.

Alternate Names

Cadherin11, CDH11, CDHOB, OB-Cadherin

Entrez Gene IDs

1009 (Human); 12552 (Mouse)

Gene Symbol

CDH11

UniProt

P55288

Additional Cadherin-11 Products

Product Documents for Recombinant Mouse Cadherin-11 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse Cadherin-11 Fc Chimera Protein, CF

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