Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF
R&D Systems, part of Bio-Techne | Catalog # 9789-SE
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Cathepsin A/Lysosomal Carboxypeptidase A protein
Ala24-Tyr474, with a C-terminal 10-His tag
Ala24-Tyr474, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala24
Predicted Molecular Mass
53 kDa
SDS-PAGE
52-60 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >160 pmol/min/μg, as measured under the described conditions.
The specific activity is >160 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
9789-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cathepsin A/Lysosomal Carboxypeptidase A
Cathepsin A/lyososomal carboxypeptidase A is a member of the serine carboxypeptidase family (1). Cathepsin A, also known as protective protein, is synthesized as a single-chain precursor and processed into heavy (32 kDa) and light (20 kDa) chains, which are linked by disulfide bonds. Cathepsin A is a multifunctional enzyme that expresses deaminidase and esterase activities at neutral pH and carboxypeptidase activity at acidic pH (2, 3). Cathepsin A is capable of hydrolyzing a variety of bioactive peptide hormones including endothelin and bradykinin making it a promising target in heart failure (2-5). A hereditary variant of cathepsin A results in cathepsin A-related arteriopathy with strokes and leukoencephalapathy (CARASAL) (6). In addition, Cathepsin A's association with beta-galactosidase ( beta-gal) and neuraminidase in a complex is essential for beta-gal stability and neuraminidase activation in the lysosomes. Inherited deficiency of Cathepsin A causes Galactosialidosis, a lysosomal storage disorder, characterized by a combined secondary deficiency of beta-gal and neuraminidase (7, 8).
References
- Pshezhetsky, A.V. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) p. 1923, Academic Press, San Diego.
- Hiraiwa, M. (1999) Cell. Mol. Life. Sci. 56:894.
- Schreuder, H.A. et al. (2014) Biochem. Biophys. Res. Commun. 445:451.
- Ruf, S. et al. (2013) Future Med. Chem. 5:399.
- Timur, Z.K. et al. (2016) Front. Mol. Biosci. 3:68.
- Bugiani, M. et al. (2016) Neurology 84:1777.
- Caciotti, A. et al. (2013) Ophanet. J. Rare Dis. 8:114.
- Annunziata, I. et al. (2017) Expert Opin. Orphan Drugs 5:131.
Alternate Names
CTSA, Lysosomal Carboxypeptidase A
Gene Symbol
CTSA
UniProt
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Product Documents for Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF
Product Specific Notices for Recombinant Mouse Cathepsin A/Lysosom Carboxypeptidase A, CF
For research use only
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