Recombinant Mouse CD229/SLAMF3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2555-CD

R&D Systems, part of Bio-Techne
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2555-CD-050
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Key Product Details

Source

NS0

Accession #

AAH95921

Conjugate

Unconjugated

Applications

Bioactivity

View all CD229/SLAMF3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD229/SLAMF3 protein
Lys48-Phe454, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys48

Predicted Molecular Mass

46 kDa

SDS-PAGE

60-80 kDa, reducing conditions

Activity

Measured by its ability to enhance anti-CD3-induced proliferation of mouse CD3+ T cells.
The ED50 for this effect is 0.5-2.5 μg/mL.

Formulation, Preparation and Storage

2555-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD229/SLAMF3

CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CD229 consists of a 406 aa extracellular domain (ECD) with two Ig‑like V‑set and two Ig‑like truncated C2‑set domains, a 21 aa transmembrane segment, and a 180 aa cytoplasmic domain with two immunoreceptor tyrosine‑based switch motifs ITSMs (2, 3). The ECD of mouse CD229 shares 59% and 73% aa sequence identity with human and rat CD229, respectively. Within the first two Ig‑like domains that are common to all SLAM proteins, mouse CD229 shares 22% ‑ 36% aa sequence identity with mouse 2B4, BLAME, CD2F‑10, CD84, CRACC, NTB‑A, and SLAM. CD229 is expressed on T, B, and NK cells, thymocytes, and monocytes (2 ‑ 7). Homophilic binding between CD229 molecules is mediated by the N‑terminal Ig‑like domain (8). Human and mouse CD229 exhibit cross‑species binding (8). Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell ‑ B cell contact (8). Y470 and Y606 in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP‑2 (μ2 chain) and GRB‑2, respectively, and both tyrosines are required for CD229 internalization (9, 10). The two ITSMs, which include Y558 and Y581, mediate phosphorylation‑dependent CD229 association with SAP and SHP‑2 (11). Antibody ligation of CD229 can inhibit T cell activation, but CD229 knockout mice show impaired T cell immune responses, suggesting a potential role for CD229 in T cell activation or co‑stimulation (7, 12).

References

  1. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
  2. de la Fuente, M.A. et al. (2001) Blood 97:3513.
  3. Sandrin, M.S. et al. (1992) J. Immunol. 149:1636.
  4. Romero, X. et al. (2004) Tissue Antigens 64:132.
  5. Hogarth, P.M. et al. (1980) Immunogenetics 11:65.
  6. Mathieson, B.J. et al. (1980) J. Immunol. 125:2127.
  7. Sintes, J. et al. (2007) Tissue Antigens 70:355.
  8. Romero, X. et al. (2005) J. Immunol. 174:7033.
  9. Del Valle, J.M. et al. (2003) J. Biol. Chem. 278:17430.
  10. Martin, M. et al. (2005) J. Immunol. 174:5977.
  11. Sayos, J. et al. (2001) Blood 97:3867.
  12. Graham, D.B. et al. (2006) J. Immunol. 176:291.

Long Name

SLAM Family Member 3

Alternate Names

CD229, Ly9, SLAMF3

Entrez Gene IDs

4063 (Human); 17085 (Mouse)

Gene Symbol

LY9

UniProt

AAH95921

Additional CD229/SLAMF3 Products

Product Documents for Recombinant Mouse CD229/SLAMF3 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Mouse CD229/SLAMF3 Protein, CF

For research use only

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