Recombinant Mouse CD2F-10/SLAMF9 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2829-CD

R&D Systems, part of Bio-Techne
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2829-CD-050
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Key Product Details

Source

NS0

Accession #

Q9D780

Conjugate

Unconjugated

Applications

Bioactivity

View all CD2F-10/SLAMF9 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD2F-10/SLAMF9 protein
Phe18-Leu230, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe18

Predicted Molecular Mass

24.5 kDa

SDS-PAGE

36-60 kDa, reducing conditions

Activity

Meassure by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3+ T cells.
The ED50 for this effect is 0.4-2 μg/mL.

Formulation, Preparation and Storage

2829-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD2F-10/SLAMF9

The CD2 family of receptors is a subset of the immunoglobulin superfamily of glycoproteins. The CD2F‑10 protein, also known as SF2001 and CD85‑H1, was identified as a CD2 subfamily member using genome sequence data. Based on its structural organization, CD2F‑10 belongs to the SLAM (Signaling Lymphocyte Activation Molecule) subfamily of CD2 receptors and is designated SLAMF9. SLAM proteins function as co‑receptors for lymphocyte activation and/or adhesion (1). Mature mouse CD2F‑10 is a type I transmembrane glycoprotein that consists of a 215 amino acid (aa) extracellular domain (ECD) with one Ig‑like V‑set and one Ig‑like C2‑set domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2 ‑ 4). Unlike other SLAM proteins, the cytoplasmic domain of CD2F‑10 does not contain consensus immunoreceptor tyrosine‑based switch motifs (ITSMs) that mediate interactions with the signal transduction proteins SAP or EAT‑2 (1). Within the ECD, mouse CD2F‑10 shares 58% and 83% aa sequence identity with human and rat CD2F‑10, respectively. It shares 21% ‑ 38% aa sequence identity with comparable regions of mouse 2B4, BLAME, CD48, CD84, CD229, CRACC, NTB‑A, and SLAM. CD2F‑10 is expressed in hematopoietic tissues and cell lines (3). R&D in house data indicate that CD2F‑10 costimulates the secretion of IFN‑ gamma from activated mouse T cells.

References

  1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol.  29:665.
  2. Fennelly, J.A. et al. (2001) Immunogenetics 53:599.
  3. Zhang, W. et al. (2001) Clin. Cancer Res. 7:822s.
  4. Fraser, C.C. et al. (2002) Immunogenetics 53:843.

Long Name

CD2 Family 10/SLAM Family Member 9

Alternate Names

CD2F10, CD84-H1, SF2001, SLAMF9

Entrez Gene IDs

89886 (Human); 98365 (Mouse)

Gene Symbol

SLAMF9

UniProt

Q9D780

Additional CD2F-10/SLAMF9 Products

Product Documents for Recombinant Mouse CD2F-10/SLAMF9 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD2F-10/SLAMF9 Protein, CF

For research use only

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