Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2580-LM

R&D Systems, part of Bio-Techne
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2580-LM-050
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Key Product Details

Source

NS0

Accession #

Q3U497

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD300b/LMIR5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CD300b/LMIR5 protein
Mouse CD300b
(Ile18-Tyr157)
Accession # Q3U497		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile18

Predicted Molecular Mass

43.2 kDa (monomer)

SDS-PAGE

55-58 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
The ED50 for this effect is 0.3-1.8 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

2580-LM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300b/LMIR5

LMIR5, also known as CD300b, CD300LB, CLM-7, and IREM‑3, is a 26 kDa-32 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse LMIR5 consists of a 140 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2). Within the ECD, mouse LMIR5 shares 51% and 86% aa sequence identity with human and rat LMIR5, respectively. The transmembrane segment contains a positively charged lysine which enables the association of LMIR5 with DAP12, DAP10, and potentially other adaptor proteins (2, 3). The cytoplasmic domain of human LMIR5 contains a phosphorylatable tyrosine motif, while that of mouse LMIR5 does not (2). LMIR5 is expressed on the surface of myeloid lineage cells (2-4). It forms noncovalent cis‑homodimers and cis-heterodimers with other CD300 family proteins, and the composition of these dimers affects the cellular response (2, 5). Antibody cross‑linking of LMIR5 induces mast cell granule release and cytokine production as well as its tyrosine phosphorylation of LMIR5 (in human) (2, 3). LMIR5 interacts with TIM1 and TIM4 which regulate T cell activation and are themselves binding partners (6). TIM1 interactions with LMIR5 mediate mast cell activation and the accumulation of neutrophils at sites of TIM1 up‑regulation on damaged renal tubule epithelial cells (6).

References

  1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
  2. Martinez-Barriocanal, A. et al. (2006) J. Immunol. 177:2819.
  3. Yamanishi, Y. et al. (2008) Blood 111:688.
  4. Wu, Y. et al. (2011) Cell. Immunol. 268:68.
  5. Martinez-Barriocanal, A. et al. (2010) J. Biol. Chem. 285:41781.
  6. Yamanishi, Y. et al. (2010) J. Exp. Med. 207:1501.

Long Name

Leukocyte Mono Ig-like Receptor 5

Alternate Names

CD300b, CD300LB, CLM7, Gm253, LMIR5, TREM-5

Entrez Gene IDs

124599 (Human); 217304 (Mouse)

Gene Symbol

CD300LB

UniProt

Q3U497

Additional CD300b/LMIR5 Products

Product Documents for Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD300b/LMIR5 Fc Chimera Protein, CF

For research use only

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