Recombinant Mouse CD300c Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10183-LM

R&D Systems, part of Bio-Techne
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10183-LM-050
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Key Product Details

Source

CHO

Accession #

A2A7V7

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD300c Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CD300c protein
Mouse CD300c
(His22-Pro187)
Accession # A2A7V7		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His22

Predicted Molecular Mass

45 kDa

SDS-PAGE

55-70 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.
The ED50 for this effect is 0.8-4.8 μg/mL.

Scientific Data Images for Recombinant Mouse CD300c Fc Chimera Protein, CF

Recombinant Mouse CD300c Fc Chimera (Catalog # 10183-LM) inhibits anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.8-4.8 μg/mL.
Recombinant Mouse CD300c Fc Chimera Protein SDS-PAGE

Recombinant Mouse CD300c Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse CD300c Fc Chimera (Catalog # 10183-LM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-70 kDa and 110-140 kDa, respectively.

Formulation, Preparation and Storage

10183-LM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300c

CMRF-35-like molecule-6 (CLM-6), known as LMIR8, CMRF-35A1, and CD300c, is a type I transmembrane glycoprotein belonging to the immunoregulatory signaling (IRS) family of immunoglobulin-like molecules (1). There have been 7 human and 9 mouse CD300/CLM family members identified, and they modulate a broad and diverse array of immune cell processes via their paired activating and inhibitory receptor functions (2, 3). Mouse CD300c consists of an extracellular domain (ECD) with one Ig-like domain, a single transmembrane segment, and a short cytoplasmic tail (2). The mature ECD of mouse CD300c is 167 amino acids and shares 48% sequence identity with human CD300c. A charged amino acid residue in the transmembrane domain of CD300c/CLM-6, found in additional family members CLM-2, CLM-4, CLM-5, and CLM-7, enables interactions with adapter proteins (3,4). CD300c has been shown to be a FcR gamma-coupled receptor that is selectively expressed in Plasmacytoid dendritic cells (pDCs) (5). CD300c signals might negatively regulate the activation of pDCs in response to viral infections (5). Additional research into CD300 receptor function during viral infections could help develop novel anti-viral therapies (6).

References

  1. Clark, G.J. et al. (2001) Tissue Antigens 57:415.
  2. Borrego F. (2013) Blood 121:1951.
  3. Vitallé J. et al. (2019) Eur J Immunol. 49(3):364.
  4. Clark, G.J. et al. (2009) Trends in Immunology 30:209.
  5. Kaitani, A. et al. (2018) Scientific Reports 8:8259.
  6. Graziano, V et al. 2019. Viruses. 11(6):498.

Long Name

CD300 Antigen-Like Family Member C

Alternate Names

CLM-6, CLM6, CMRF35, IGSF16

Entrez Gene IDs

10871 (Human); 387565 (Mouse)

Gene Symbol

CD300C

UniProt

A2A7V7

Additional CD300c Products

Product Documents for Recombinant Mouse CD300c Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse CD300c Fc Chimera Protein, CF

For research use only

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