Recombinant Mouse CD39L2/ENTPD6 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6288-EN

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

NP_742115

Conjugate

Unconjugated

Applications

Enzyme Activity

View all CD39L2/ENTPD6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CD39L2/ENTPD6 protein
Lys33-Leu455, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

47 kDa

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate guanosine-5’-diphosphate (GDP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >80,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6288-EN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: CD39L2/ENTPD6

CD39L2, also known as ectonucleoside triphosphate diphosphohydrolase-6 (ENTPD-6) and NTPDase-6, is a nucleoside phosphohydrolase of the CD39 family of enzymes that is present on the surface of cells (1). This Type II membrane protein also exists as a soluble, secreted protein. CD39L2 displays a preference for the nucleoside-5’-diphosphates GDP and IDP over CDP and UDP (2). Nucleoside-5’-triphosphates are also hydrolyzed, but at much lower rates. CD39L2 is expressed at its highest levels in cardiac muscle and in heart capillary endothelial cells (3). CD39L2 may play a functional role in the regulation and recruitment of platelet activation in the heart. The cytoplasmic and transmembrane domains of rmCD39L2 were replaced with a signal sequence, resulting in the secretion of the recombinant protein.

References

  1. Chadwick, B.P. and A.M. Frischauf (1998) Genomics. 50:357.
  2. Hicks-Berger, C.A. et al. (2000) J.Biol. Chem. 275:34041.
  3. Yeung, G. et al. (2000) Biochemistry. 39:12916.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 6

Alternate Names

CD39L2, ENTPD6, NTPD3, NTPDase 6

Entrez Gene IDs

955 (Human); 12497 (Mouse); 85260 (Rat)

Gene Symbol

ENTPD6

UniProt

NP_742115

Additional CD39L2/ENTPD6 Products

Product Documents for Recombinant Mouse CD39L2/ENTPD6 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CD39L2/ENTPD6 Protein, CF

For research use only

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