Recombinant Mouse CD39L3/ENTPD3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4464-EN
Key Product Details
Source
CHO
Accession #
Structure / Form
Monomer
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived mouse CD39L3/ENTPD3 protein
Gln44-Pro485, with a C-terminal 6-His tag
Gln44-Pro485, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained, Gln44 predicted.
Predicted Molecular Mass
50 kDa
SDS-PAGE
85-100 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >20,000 pmol/min/μg, as measured under the described conditions.
The specific activity is >20,000 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
4464-EN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD39L3/ENTPD3
Ectonucleoside triphosphate diphosphohydrolase-3 (NTPDase-3) is an integral membrane protein with an extracellular catalytic domain (1). rhNTPDase-3 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble rhNTPDase-3 ectodomain. NTPDase-3 hydrolyzes the b- and g phosphate groups of nucleotides, preferring ATP, ADP, UTP, and UDP as substrates (1). Through its hydrolysis of extracellular nuceotides, NTPDase-3 is important for the regulation of purinergic signaling (2). The enzyme is expressed at its highest levels in brain, pancreas, spleen, and prostate tissues (3). In the brain, NTPDase-3 may play a role in the regulation of feeding, sleep, and other behaviors (4).
References
- Lavoie, E.G. et al. (2004) Biochem. Pharmacol. 67:1917.
-
Crawford, P.A. et al. (2007) Arch. Biochem. Biophys. 457:7.
- Chadwick, B.P. and A.M. Frischauf (1998) Genomics 50:357.
- Belcher, S.M. et al. (2006) Neuroscience 137:1331.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 3
Alternate Names
ATPDase, CD39L3, ENTPD3, HB6, NTPDase-3
Gene Symbol
ENTPD3
UniProt
Additional CD39L3/ENTPD3 Products
Product Documents for Recombinant Mouse CD39L3/ENTPD3 Protein, CF
Product Specific Notices for Recombinant Mouse CD39L3/ENTPD3 Protein, CF
For research use only
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