Recombinant Mouse Cerberus 1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1986-CB/CF
Key Product Details
Source
E. coli
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
E. coli-derived mouse Cerberus 1 protein
Asp18-Pro272, with an N-terminal Met
Asp18-Pro272, with an N-terminal Met
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
28.7 kDa (monomer)
SDS-PAGE
34 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
In a 100 µL reaction mixture containing rmCerberus 1 at 1 µg/mL and rhBMP-4 (Catalog # 314-BP) dilutions at 0.02-3 µg/mL, the concentration of
rhBMP-4 that produces 50% of the optimal binding response is found to be approximately 0.2-0.8 µg/mL.
In a 100 µL reaction mixture containing rmCerberus 1 at 1 µg/mL and rhBMP-4 (Catalog # 314-BP) dilutions at 0.02-3 µg/mL, the concentration of
rhBMP-4 that produces 50% of the optimal binding response is found to be approximately 0.2-0.8 µg/mL.
Formulation, Preparation and Storage
1986-CB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCI. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cerberus 1
Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1, 2). Mature mouse Cerberus 1 shares 40%, 29%, and 66% amino acid (aa) sequence identity with chick, Xenopus, and human Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24% - 37% aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms disulfide-linked homodimers (3). Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain (4). At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh (3, 5 - 9). Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation (3, 4, 6, 8, 10 - 12). In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction (4 - 8, 13).
References
- Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
- Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
- Biben, C. et al. (1998) Dev. Biol. 194:135.
- Piccolo, S. et al. (1999) Nature 397:707.
- Zhu, L. et al. (1999) Curr. Biol. 9:931.
- Tavares, A.T. et al. (2007) Development 134:2051.
- Foley, A.C. et al. (2007) Dev. Biol. 303:57.
- Bouwmeester, T. et al. (1996) Nature 382:595.
- Bertocchini, F. and C.D. Stern (2002) Dev. Cell 3:735
- Glinka, A. et al. (1997) Nature 389:517.
- Perea-Gomez, A. et al. (2002) Dev. Cell 3:745.
- Takaoka, K. et al. (2007) Curr. Opin. Genet. Dev. 17:344.
- Simpson, E.H. et al. (1999) Dev. Biol. 213:202.
Alternate Names
CER1, DAND4
Gene Symbol
CER1
UniProt
Additional Cerberus 1 Products
Product Documents for Recombinant Mouse Cerberus 1 Protein, CF
Product Specific Notices for Recombinant Mouse Cerberus 1 Protein, CF
For research use only
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