Recombinant Mouse Chondroadherin His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10033-CH

R&D Systems, part of Bio-Techne
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10033-CH-050
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Key Product Details

Source

NS0

Accession #

O55226

Conjugate

Unconjugated

Applications

Bioactivity

View all Chondroadherin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Chondroadherin protein
Ala21-Thr349, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Protein identity confirmed via Mass Spectrometry.

Predicted Molecular Mass

38 kDa

SDS-PAGE

33-38 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.08-048 μg/mL

Scientific Data Images for Recombinant Mouse Chondroadherin His-tag Protein, CF

Recombinant Mouse Chondroadherin His-tag Protein Bioactivity

Recombinant Mouse Chondroadherin His-tag Protein Bioactivity

Recombinant Mouse Chondroadherin (Catalog # 10033-CH) induces adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.08-0.48 μg/mL.
Recombinant Mouse Chondroadherin His-tag Protein SDS-PAGE

Recombinant Mouse Chondroadherin His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Chondroadherin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 33-38 kDa.

Formulation, Preparation and Storage

10033-CH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Chondroadherin

Chondroadherin (CHAD), also known as SLRR4A, is a 38 kDa secreted protein belonging to the small leucine-rich proteoglycans (SLRPs) that help regulate the assembly and function of the extracellular matrix (ECM) (1). CHAD is highly expressed in cartilaginous tissues, with lower expression levels found in bone, tendon, and eye (2-4). Mature mouse CHAD is a 338 amino acid (aa)  protein that contains twelve leucine-rich repeats (LRRs) including ten tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains flanked by cysteine-rich regions. CHAD interacts with collagen II and mediates signaling between chondrocytes and the ECM by binding to the alpha2 beta1 integrin, heparan sulphate, and to cell surface proteoglycans like syndecans (5-9). In addition, CHAD also interacts with both N- and C-terminal globular domains of type VI collagen (10). Mouse CHAD shares 95% and 98% aa sequence identity with human and rat CHAD, respectively.

References

  1. Neame, P.J. et al. (1994) J. Biol. Chem. 269:21547.
  2. Larson, T. et al. (1991) J. Biol. Chem. 266:20428.
  3. Mizuno, M. et al. (1996) Calcif. Tissue Int. 59:163.
  4. Johnson, J.M. et al. (2006) Mol. Vis. 12:1057.
  5. Månsson, B. et al. (2001) J. Biol. Chem. 276:32883.
  6. Paracuellos, P. et al. (2017) Matrix Biol. 63:106.
  7. Batista, M. et al. (2014) Matrix Biol. 38:84.
  8. Camper, L. et al. (1997) J. Cell Biol. 138:1159.
  9. Haglund, L. et al. (2013) J. Biol. Chem. 288:995.
  10. Wiberg, C. et al. (2002) J. Biol. Chem. 277:49120.

Alternate Names

CHAD, SLRR4A

Entrez Gene IDs

1101 (Human); 12643 (Mouse); 29195 (Rat)

Gene Symbol

CHAD

UniProt

O55226

Additional Chondroadherin Products

Product Documents for Recombinant Mouse Chondroadherin His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse Chondroadherin His-tag Protein, CF

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