Recombinant Mouse CHST3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5356-ST

R&D Systems, part of Bio-Techne
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5356-ST-020
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Key Product Details

Source

CHO

Accession #

NP_058083

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Carbohydrate Sulfotransferase 3/CHST3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Carbohydrate Sulfotransferase 3/CHST3 protein
Glu39-Thr472, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

50 kDa

SDS-PAGE

60-80 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to chondroitin sulfate.
The specific activity is >350 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

5356-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Carbohydrate Sulfotransferase 3/CHST3

The mouse CHST family is comprised of 13 enzymes. All members of this family are Golgi‑localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST3, also known as chondroitin 6-O-sulfotransferase, transfers sulfate to position 6 of GalNAc residues on chondroitin sulfate (3). Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Loss of CHST3 function in human results in severe chondrodysplasia (4). CHST3 can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides (5). Mouse CHST3 shares 85% amino acid sequence identity to the human ortholog.

References

  1. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
  2. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
  3. Uchimura, K. et al. (2002) J. Biol. Chem. 277:1443.
  4. Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. U. S. A. 101:10155.
  5. Yusa, A. et al. (2006) J. Biol. Chem. 281: 20393.
    6.  

Alternate Names

C6ST1, CHST3, GST-0

Entrez Gene IDs

9469 (Human); 53374 (Mouse); 84468 (Rat)

Gene Symbol

CHST3

UniProt

NP_058083

Additional Carbohydrate Sulfotransferase 3/CHST3 Products

Product Documents for Recombinant Mouse CHST3 Protein, CF

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Product Specific Notices for Recombinant Mouse CHST3 Protein, CF

For research use only

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