Recombinant Mouse CLEC4F/CLECSF13 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2784-CL
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse CLEC4F/CLECSF13 protein
Ala65-Gly548, with an N-terminal 9-His tag
Ala65-Gly548, with an N-terminal 9-His tag
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
55 kDa
SDS-PAGE
65-85 kDa, reducing conditions
Activity
Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles.
The ED50 for this effect is 0.75-3.75 µg/mL.
The ED50 for this effect is 0.75-3.75 µg/mL.
Formulation, Preparation and Storage
2784-CL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CLEC4F/CLECSF13
CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1 - 3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N-acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).
References
- Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
- Bilzer, M. et al. (2006) Liver Int. 26:1175.
- Kuiper, J. et al. (1994) Biochem. J. 299:285.
- Accession # P70194.
- Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
- Fadden, A.J. et al. (2003) Glycobiology 13:529.
- Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
- Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
- Coombs, P.J. et al. (2006) Glycobiology 16:1C.
- Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
- Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.
Long Name
C-type Lectin Domain Family 4, Member F
Alternate Names
CLECSF13, Galactose Particle Receptor, KCLR, KCR
Gene Symbol
CLEC4F
UniProt
Additional CLEC4F/CLECSF13 Products
Product Documents for Recombinant Mouse CLEC4F/CLECSF13 Protein, CF
Product Specific Notices for Recombinant Mouse CLEC4F/CLECSF13 Protein, CF
For research use only
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