Recombinant Mouse Corneodesmosin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8250-CN

R&D Systems, part of Bio-Techne
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8250-CN-050
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Key Product Details

Source

NS0

Accession #

AAH55373

Conjugate

Unconjugated

Applications

Bioactivity

View all Corneodesmosin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Corneodesmosin protein
Lys33-Ser561, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys33

Predicted Molecular Mass

52 kDa

SDS-PAGE

60-75 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells.
The ED50 for this effect is 1.5-7.5 µg/mL.

Formulation, Preparation and Storage

8250-CN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Corneodesmosin

Corneodesmosin, also known as CDSN and the S gene product, is a highly polymorphic secreted glycoprotein that plays an important structural role in the skin (1). Mature mouse Corneodesmosin shares 62% and 82% amino acid sequence identity with human and rat Corneodesmosin, respectively. It is expressed by differentiated keratinocytes in the corneal layer of the skin and is a major component of corneodesmosomes (2-4). It is also expressed in the inner root sheath of hair follicles (5, 6). Corneodesmosome has a high content of glycine, serine, and proline residues that promote its folding into a series of Gly-loop domains (2, 7). Corneodesmosin forms oligomers and associates homophilically to strengthen the adhesion between corneocytes (8, 9). Corneodesmosin-deficient mice exhibit a detachment of the corneal layer of the skin as well as hypotrichosis of the scalp and baldness (6, 10). Corneodesmosin is secreted by keratinocytes as a 52-56 kDa molecule which is then subjected to repeated sequential N- and C-terminal proteolysis (11). Species of 46, 43, 36, and 15 kDa are present in corneocytes (7, 11). Cleavage of the N-terminal Gly-loop diminishes Corneodesmosin’s ability to mediate adhesion, and this is a prerequisite for normal desquamation of the skin (8, 9). Reduced proteolysis of Corneodesmosin in psoriasis lesions is associated with the persistence of corneodesmosomes and scale retention (12). Premature truncation of Corneodesmosin is associated with hypotrichosis of the scalp (13).

References

  1. Jonca, N. et al. (2011) Eur. J. Dermatol. 21 Suppl. 2:35.
  2. Zhou, Y. and D.D. Chaplin (1993) Proc. Natl. Acad. Sci. 90:9470.
  3. Haftek, M. et al. (1997) Br. J. Dermatol. 137:864.
  4. Simon, M. et al. (1997) J. Biol. Chem. 272:31770.
  5. Mils, V. et al. (1992) J. Histochem. Cytochem. 40:1329.
  6. Matsumoto, M. et al. (2008) Proc. Natl. Acad. Sci. 105:6720.
  7. Guerrin, M. et al. (1998) J. Biol. Chem. 273:22640.
  8. Jonca, N. et al. (2002) J. Biol. Chem. 277:5024.
  9. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:747.
  10. Leclerc, E.A. et al. (2009) J. Cell Sci. 122:2699.
  11. Simon, M. et al. (2001) J. Biol. Chem. 276:20292.
  12. Simon, M. et al. (2008) Br. J. Dermatol. 159:77.
  13. Levy-Nissenbaum, E. et al. (2003) Nat. Genet. 34:151.

Alternate Names

CDSN, D6S586E, HTSS

Entrez Gene IDs

1041 (Human); 386463 (Mouse)

Gene Symbol

CDSN

UniProt

AAH55373

Additional Corneodesmosin Products

Product Documents for Recombinant Mouse Corneodesmosin Protein, CF

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Product Specific Notices for Recombinant Mouse Corneodesmosin Protein, CF

For research use only

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